LOCUS       sequence-234477-        8364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_090100-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3522..3539
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3591..3646
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3645..3664)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5882..5900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5968..6072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6073..6141,6142..6933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6291..6310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7104..7692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7593..7612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7846..7863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7980..8001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8016..8021,8022..8039,8040..8046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8054..8070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8059..8081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8078..8094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8078..8094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgagtaca
     1201 tctgccagat catgcccaac atcagcatga acttcggcat cctgggcgag cccatcgaca
     1261 agcggcagta cggcggcaac cggctgaagt tcatcgtgat ctacagagag agcatccagc
     1321 ggcacagcct gttcggccgg ttcaagagcg agatcgtgga aaaggccctg aacttctggg
     1381 agcggaccct gagcgtgcgg aggcccccca acagaaagct gctgatcgac cggggctgcg
     1441 tggaacccgt gttctacacc gacggccgga ccggcaagaa gttctgcaag agccagtgca
     1501 agcagaaagc cctgtgctac gaccaccccg tgcccgacca gtacgcctct ggatgtgcta
     1561 tgggcggagg cggcggagac atccgggacg tgtaccagga tggccctggc ttcgccccca
     1621 acgagtacgt gctgttcgtg gaaagcgaga acaagcaggg ctgcctgagc ggcgccacac
     1681 tggcctatgc cggcccttgc gagatgcacc ccaccaccga ccggcccatc atgggcagca
     1741 tcaacttttg tccacaaaag atggaaatcc aggaacccgg caagaccatg ctgatcggca
     1801 ccgccatcca cgagctgggc cacgccctgg gcttcgtgaa gtccaacttc gccctgatgc
     1861 gggaccccga gggcaacccc agaaccccca gagatcccaa gagcggcaga ccccccctga
     1921 acaacgagag acagtacagc gccagcgaga acaccgtgcg gatcatcaac cggccctggg
     1981 tcaccgccgc tggcaccttc agcaagccct tcatgagctt cgtgacccct gccctgctgg
     2041 aagaggcccg gaagcacttc aactgcccca acctggacgg cctggacatc gagaatgagg
     2101 gcggccaggg caccatcggc acccacttcg agaagcgggt cgtgggcgac gagacaatgg
     2161 ccggcgtgac aggcgtgaag tccgtgctga gccggctgac cctggccttc ttcaccgaca
     2221 gcgggtggtg ggacgtggac tacagcctgg ccgagccctg gtactacggc aagaacctgg
     2281 gctgcagctt cgtgatggaa agctgctacg cctacatgat gcggatgaag caggccggca
     2341 agagcaccga gccctactgc gacgagcccg acaccctgaa gtgctaccac cagaaggcct
     2401 tcggcatctg cgccgtgggc cggttcaccc agagactgcc ccccaccgag cagtacttca
     2461 acgaccctac ccagggcggc accagcgtgc tgaacgacag atgccccctg atccagccta
     2521 tgcggagctt cttcaacgag cccctggtca cctactgcga tcaccagctg aacatccccg
     2581 tggcccagcg gggcaacatg ttcgcccagg acttcggcaa caacagcatg tgcatcatcc
     2641 acaagggcgc ctggcgggct gagatgaacg gcagagccac caacgacccc agagtgaagg
     2701 ccacctgtca ccagtacagc tgctctggcg gcctgcaggt catcatcaac ggcaagcctt
     2761 tccagtgcaa cagcggcgtg gccaagatcc agaccaagca gatccagggc gagatcatct
     2821 gccccaatcc caatatcgcc tgccggaaca gacggcggag catcaagcaa ggcgcgccgt
     2881 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2941 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     3001 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     3061 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     3121 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     3181 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     3241 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3301 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3361 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3421 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     3481 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     3541 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     3601 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3661 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     3721 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     3781 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     3841 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3901 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3961 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     4021 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     4081 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     4141 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     4201 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     4261 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     4321 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     4381 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     4441 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     4501 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     4561 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     4621 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     4681 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     4741 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     4801 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     4861 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4921 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4981 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     5041 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     5101 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     5161 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     5221 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     5281 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     5341 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     5401 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5461 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     5521 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     5581 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5641 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5701 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5761 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5821 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     5881 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5941 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6301 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6601 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7861 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7921 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7981 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8041 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8101 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     8161 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     8221 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     8281 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     8341 catgtccaat atgaccgcca tgtt
//
