LOCUS       sequence-234484-        8034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_071610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2812..2829
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2881..2936
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2935..2954)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5172..5190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5258..5362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5363..5431,5432..6223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5581..5600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6394..6982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6883..6902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7136..7153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7270..7291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7306..7311,7312..7329,7330..7336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7344..7360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7349..7371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7368..7384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7368..7384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7655..8034
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccatccctt ggccccccaa agagaactac
      841 ttcgaccaga ccctggacca cttcaatttt caggcccgga acctgacctt caagcagaga
      901 tacctgtacg aggacaagtg gttcaagccc aacggcccca tcttcttcta ctgcggcaac
      961 gagggcggca tcgacggctt ttggaacaac accggcctga tcttcgagct ggcccccagc
     1021 ttcaacgcct tcgtgctgtt cgccgagcac cggtactacg gcaagagcct gcccttcaac
     1081 accagcttcc agcagcccta catccagtac ctgagcatcg atcaggccct ggccgactac
     1141 gcctacctga tcgagggcat caagagcacc ttcaacatga cccggtccct ggtggtggcc
     1201 ttcggcggct cttatggcgg catgctggcc gcctacatgc gggccaagta cccccacatc
     1261 atcaagggcg ccctggccag cagcgcccct gtcagatggg tggccggcga gggcaacttc
     1321 cacgacttct tcgagagcgt gaccaaggac taccgggacg ccgaccccaa gtgcagcgag
     1381 aagatcaaga acgccttcaa cctggccgtg cagctgagcc agaagcccga catcggctac
     1441 aagcagctga gcaacgacct gcggctgtgc aagcccatcc agaacgactt cgagttctac
     1501 tgggtgctga agtgggcccg gaatgccttc gtgatgatgg ccatgctgga ctacccctac
     1561 aaggccagct tcatggccag cctgcccgcc aaccccgtga acgtgtcctg caagaacgcc
     1621 ctggccgtga ccgacctgat ccccaccctg agagaagccg tgggcgtgtt ctacaacagc
     1681 agccagtccc tgccctgctt cgactacaag acccagttca tcgagtgcgc cgacatcacc
     1741 ggctgcggcc tgggctctga tagcctggcc tgggacttcc agagctgcac cgagatgaac
     1801 ctgcacgacg acagcgacag caccaccaac gacatgttcg tgtccctgcc cctgaccaag
     1861 cagcaggtca caagctactg ccagcggaga tggggcgtga cccccgcctt caatcagctg
     1921 agcaccttct acggcgacaa catctggaaa accagcagca acatcatctt cagcaacggc
     1981 aacctggacc cctggatggg cggaggcatc ctgaccgacc agagcgagaa agtgatcagc
     2041 ctggtgctgg acggcggagc ccaccacctg gacctgagaa gccccgaccc caacgacccc
     2101 cctagcgcta gacaggtccg acagatcgag gtgcagacca tcagaagctg gctggacctg
     2161 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     2221 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     2281 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     2341 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     2401 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     2461 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     2521 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     2581 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     2641 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     2701 gttttatcca aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg
     2761 ctgtggaacc acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat
     2821 caccaccatt gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct
     2881 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     2941 gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag
     3001 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     3061 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     3121 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     3181 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     3241 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     3301 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     3361 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     3421 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     3481 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     3541 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     3601 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     3661 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     3721 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     3781 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     3841 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     3901 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     3961 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     4021 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     4081 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     4141 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     4201 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     4261 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     4321 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     4381 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     4441 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     4501 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     4561 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     4621 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4681 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4741 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     4801 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     4861 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4921 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4981 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5041 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5101 ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg
     5161 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5221 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5581 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     5641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5821 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5881 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     5941 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6001 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6061 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6121 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6181 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6541 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7021 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7081 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7141 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7201 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7261 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7321 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7381 tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta
     7441 tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg
     7501 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
     7561 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
     7621 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
     7681 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     7741 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7801 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     7861 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     7921 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7981 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catg
//
