LOCUS       sequence-234487-       13014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_089670-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..13014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7792..7809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7861..7916
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7915..7934)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10152..10170)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10238..10342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10343..10411,10412..11203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10561..10580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11374..11962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11863..11882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12116..12133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12250..12271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12286..12291,12292..12309,12310..12316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12324..12340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12329..12351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12348..12364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12348..12364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        12635..13014
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccagcaccg acgtgcacat caccagcagc
      841 aacgagaacc ccttcaagac cgatgtgcag cagctgaaca tcaccaacga cagcaacaac
      901 accgagacaa tcagcagcac cctgtacccc aacgtgacct acgtgatcct gcccaccggc
      961 ttctacctgg gccagaccaa cctgatcacc gtgcggagca gagagccccg gacccagctg
     1021 cggatcagca tgaacgtgac cgagctgccc cacgagatca acgaccccga ccagatctac
     1081 tacctggaac ccctgaacac caccgagtat tggtacggca tcgatatccc ttacgagatc
     1141 cctttcatct tcacccacga ggactctatg tgggtcaagc tgatcttcca cttcacccac
     1201 tgcctgaaca agaccgacgt gatccccacc gacaacgagt gcaccaccta caagaccacc
     1261 gaagtgatcc acagcatcca gctgaagcag ccccccatga tcatcctggg cgagacagac
     1321 aagcccatct acagacccgg cgagaacgtg cggttccggt tcctgaccct gaacgtgaac
     1381 agcctgctgc ccagaagcac cagcatcccc ctgcccaaga agaagctgat cattcacaag
     1441 ggcagcaagg gccagctgat cgagatgaac aagctggaac tgatgaagtg gaacaacatc
     1501 atctacgact ccatcgtgat catcgacccc atggacaacc acgtgaagca gtggttcaac
     1561 atcagcccca accaggccat caacctgacc taccccatcc tgcacaacgc caaagagggc
     1621 cagtggaagc tgcgggccac cgtgcggcac cactacatcg agacaatcga gttcacagtg
     1681 aagcagtaca ccctgcctaa gttcaccgtg tccctggaaa cccccagagt gttcaacatg
     1741 gaaagcgagt acgtgcagta cagcgcctgc gccaagtaca ccaacggccc acccctgaag
     1801 ggccacatcc agagcatcct gtgcgcctgc accgactaca tctgggacaa ccacttccac
     1861 cagctggacg acgagcagat cgtgcagagc ctgctgacca accccaagtg ccccagcgac
     1921 ggctacgaga caaagacccg gccctgcatc atcatcaacc agcccctggc ccccaacggc
     1981 tgcaccaact tcaacatctc taccaccgag ttcgccctgt tcagcagcaa gtacagcaag
     2041 tggaaccaga tctccatcct gtgcacccag gtcatcgagg acgacaccgg ctccaagctg
     2101 ttcaactgca tcaagggcga ccccatcaag atgaaccagc tgagcctgca catcgagctg
     2161 cctcacgtgt acaagagcaa gctgcccatc accggcaaag tgaagctgct gaactacgac
     2221 aagaacacca actacagcgt gaagatcagc gtgtccgacc agaagtgggg ctgctactgg
     2281 cagaacggca acaccgacaa ccagcactac ctgaatgtga tctccatgaa cagcaacggc
     2341 gagggcctga tctacctgcc ccccatcagc agccagcaca gcatcagcgt ggaagccgag
     2401 ctgctgcaga gcagcgtgtc cagcacccgg tccagcctga acagcagcac cagctccaac
     2461 gaaaccacca gcaacaccac caaccagaac agcccccgga agctgaccaa cgagaagccc
     2521 agcagcctgt actactggcc ccaccggccc tggatcaacg atcaccggat catcgacaac
     2581 gtggtgctgc ggagctggga cagcatctcc cagatctatc tgcagctgtg gccccctcac
     2641 gacaaagtga tcaccacctg tcccaacctc gtgaagctga ccctgctgag caacacccgg
     2701 ctgagcgaca aggccatctt catcgagagc gtgatccggg gcaagcacca gaaaatcgtg
     2761 atccctgccg acagcatcga caatgacaac gactactgca tcgaccgcga cgacgagctg
     2821 ggccactaca agtgcctgaa ctggaacagc accgagatcg agtgcctgcc cggctggaac
     2881 ggcgagaact gcctgatccc cgtgtgcagc ctggaatgca gcaagaaggg cggcttctgc
     2941 agcaagccca accagtgcca gtgcaagacc ggctggaccg gcatcacctg tgaccagtgc
     3001 gtgaagagag agaattgcct gcacggcaag tgcctgctgg gcaacgactg catctgcgag
     3061 cctggctggg ccggctacct gtgcgacagc aagaaagtga tctacgagaa gatcgaggat
     3121 gacagcgaga caaactccac cgacagcgtg accgaggaaa tcaacaacga ggacagcgag
     3181 aagcacagca ccatgaactc ccccagccag atcaacacca agcccatccg gaccctgtac
     3241 cagcggtaca tccagttcaa gatcgacggc agctggggcc ccaagttcac cgccgtgctg
     3301 tacttccaca atcagcacga cgtgaccatc cccgagatca tcagcaccca gatcaccatc
     3361 gagcagattg acaactgctc cagcaacgcc attgccctgg aaagcaagag caaccacacc
     3421 aacctgaagc tgagcaagaa gatcgccgac cccggcgagc agatcaagat gacaatcatc
     3481 ccccagggcg tgtcccagtg gatggaaaag tccaccagct acgacatgaa gtccctgaac
     3541 tccaacggcg acaacaacaa cgacaaggga cagctgagca acgagctgtg cttcgtgcgg
     3601 atctccgaca tcagcctgga caacttccag ggcgacaaga acctggtcaa cctgaagtac
     3661 tttaccgaca agctgattga gttcaccggc gagagcggct accggcccct ggtggccagc
     3721 tccacccagg aagcctttct ggccgctggc ttccagctgg gcagcaccta ccccgagccc
     3781 accttcgtgc acagagtgta cccctgcccc tacctggcct acagcaacgc cctgcccgtg
     3841 gtcatggacg atgctgtggg cgacggcgag tacaagtcca acagcgagca ccggcagacc
     3901 atcagcctgc ggaagggcct gcacgacacc atcaagcccc ggctgcggga cttcttccca
     3961 gaagtctggc tgttcgacgt gatgcctatc accaacctgc agaacaacga cgccgacgac
     4021 tacaacgaga acgacatcga gaacaccggc ttcaacgaga caaacaagct gaaggatatc
     4081 gagctgaacc tgaccgtgcc cgataccatc accagctggc gggccagcgc ctactgcacc
     4141 accaaagaaa acggcctgtg gatcggcgag ccccagatcc tgaccgtgac catgcccttc
     4201 tacaccgaga tcaccctgcc caaagagatg aagcggggcg agatcctgca catccccatc
     4261 agcgtgttcg tgctggaccg gaagtacctg aaccctaccg acgacaattt caacaagaca
     4321 gagtgctacg aaaccagcgt gcggatccag gtcaacaaca gcgagtggct gatcacaacc
     4381 agcaacgagt tcaccaattg cctgtgctcc ggccagaaaa tcacctacct cgtgggcctg
     4441 ctgcctcagc agctgggcca gctcaacgtg accgtggaag ccttcgccat ccggaacagc
     4501 gagttctgcg gcctgatcca gaccaacgac aacggcaaca gcctgacctt cgagagcaac
     4561 aagactgaga tgaagaccaa ccagtccaag ctgctgtccg acaagatgat ccggcagatc
     4621 aatgtgatcc ccgagggcat ccagcaggaa accaccctga gcgacatcgt gtgcctgaat
     4681 gagcagcaga ccaccagcac cagacacttc cagttcaccc tgccagacca catcatcaag
     4741 gacagcttcc acagctacat cagctacagc gacgaggtgc tgggccctgc cctcgtgaac
     4801 ctggacagcc tgatccggct gcctaccggc tgtggcgagc agaacatggt gctggtcgcc
     4861 cccaacgtgt acatcctgga ctacctgaag tccaccccca acatcggcat caacgacaac
     4921 aaagagaagt atatcgagag cgccaagagc cacatcatct ccggctacta ccagcagatg
     4981 aagtaccggc gggacgacgg ctccttcagc gccttcggca agagcgacaa agagggctcc
     5041 acctggctga ccgccttcgt gctgagagtg ttcgccaagg cctacagact gcagcccaac
     5101 ctgagcatcg actggaacaa cctgttcaac ggcgccatca actacctgat catgcaccag
     5161 aacaatgaga gcggctgctt cgaggaatac ggcaaggtgc tgtacagccc cctgcagggc
     5221 atcagcggca ccgacgagac acagtggcgg agcatcctgc tgacaagcta cgtgtccagc
     5281 gccctgctgg aaatccagag caccttcgac aaagagaacg acgagaacaa ggtcgactac
     5341 ctgcacatca agaactacac cacagagatc aagaatagct tcgacgccgt gttccggtgc
     5401 ctgaatacca agctggtgtc tctggacttc ttcaaagaaa tccccacctc cgtgctggtg
     5461 cagctgagct acacctacac catcatcaag cccaacaacg agctgaccgt gaagctgcag
     5521 aatgagacaa tcaaccggaa gcaggtcaca aacgaccagt tcggcaccaa gatcttttgg
     5581 agcagcaact actgcgagaa caacatgacc acccagaaga acaagaacga gccccgggac
     5641 ctggaatcca ccgcctacgc ctttctgatg ctgaaccaga tgaatcagtc cgtgtccgat
     5701 ctgttcccca tcatccggtg gatcagctcc cagcagaagt ccaacggcgg cttttacagc
     5761 acccaggaca ccgtgctgag cctggaagcc attgccgagt ttgccaagcg gctgggcctg
     5821 agcaagaccc tggacagcaa cgtgtcctcc aacgtgctgt tcatcagcaa caagatgtcc
     5881 ttcgtgaact acaccctgga cgatctgatc accagcgaga agagacaggt catcaatcag
     5941 attgagctgc ccagataccc cctggacgac aaccagaaga tcatcgactc cacctgggag
     6001 ctgaagtcca atgagagcca gaccgactgc atcctggtgc agaacacctt catctacaac
     6061 atgcccgaga gagaggacat cgacaagaag atcaagctga gcttcgacct ggtgcagcag
     6121 aacaagctga gcgacgtgaa ctgcaagctg gccaccctgt ccatgtgcct gcagctcaac
     6181 aacatgaaga acgtgtccac catcagcacc ggcatgctgc tgatcagagt gacaatggtc
     6241 accggctggg agcccatcct ggaagaactg cacagccagc tgggctccga ggacgagagc
     6301 ctgagaaagt tcaccatgaa cgaccagaac gagatctccc tgtacttcga cgagttcagc
     6361 gagaacgaaa cccggaaagt gggcggcgac tggaccaagc tgaagagatg cgtgtccctg
     6421 cccctgaagc agatccacta cgtggaaaac gccaagaccg ccaccatcac aggcttcgag
     6481 tactacaccc ccgacgagtc cgtgaccgtg acataccagc tggatgagtg ccggcagggc
     6541 tggaacatca gcatcgacca caccaccacc gacgacttca ccgagtccat catgaccacc
     6601 gacacctcca tccagaatat caccaccacc ccccagatga agacactgtg ccccatctgc
     6661 atcaaccagg gcaatcacac cgccctgctg cccgaggaca tcttcaccag catctgcaac
     6721 tacaccaatg gcatctacct catcaaggtg ctcgagtaca acaacaatac catgaacgtc
     6781 accatcatcc agatcctgca gtcccggtac atcacctctt ggaacaccac catcctgttc
     6841 cccaacctgc tggaatgctc ctgcaatatc atccagaccc agcagaatct ggtgctgttc
     6901 ttcgaccagt acaccaatat ctaccagggc gacgcccaga tcaatctgaa caccatcgac
     6961 accaatgtga ccctgatctc caccaacgac gtgctgccca atctgaagct ggccaatgag
     7021 aaatggctga ccatgagcaa cagcaccacc gatgacgact tcaactacta cgacctcttc
     7081 aactacaact gcaagcggct gcccctgatc gtgaactata tccaggaaaa gttcctgaac
     7141 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     7201 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     7261 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     7321 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     7381 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     7441 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     7501 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     7561 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     7621 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     7681 gttttatcca aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg
     7741 ctgtggaacc acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat
     7801 caccaccatt gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct
     7861 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     7921 gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag
     7981 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     8041 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     8101 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     8161 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     8221 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     8281 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     8341 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     8401 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     8461 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     8521 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     8581 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     8641 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     8701 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     8761 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     8821 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     8881 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     8941 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     9001 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     9061 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     9121 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     9181 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     9241 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     9301 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     9361 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     9421 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     9481 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     9541 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     9601 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     9661 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     9721 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     9781 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     9841 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9901 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9961 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
    10021 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    10081 ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg
    10141 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
    10201 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    10261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    10321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    10381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    10441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    10501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    10561 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
    10621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    10681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    10741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    10801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    10861 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
    10921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    10981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    11041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    11101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    11161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    11221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    11281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    11341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    11401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    11461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    11521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    11581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    11641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    11701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    11761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    11821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    11881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    11941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    12001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    12061 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    12121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    12181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    12241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    12301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    12361 tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta
    12421 tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg
    12481 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
    12541 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
    12601 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
    12661 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
    12721 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    12781 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
    12841 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
    12901 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
    12961 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catg
//
