LOCUS       sequence-234496-        7422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_112090-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2200..2217
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2269..2324
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2323..2342)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4560..4578)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4646..4750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4751..4819,4820..5611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4969..4988)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5782..6370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6271..6290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6524..6541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6658..6679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6694..6699,6700..6717,6718..6724)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6732..6748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6737..6759
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6756..6772
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6756..6772
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7043..7422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcctttgaga gagcctctac ctggctcgtg
      841 cggaagggcg agcccgtgca ggaccggacc gagttcccct atatcgcctt cgtgcggacc
      901 gagcggacca tgtgcaccgg cagcctggtg tccaccagag ccgtgctgac agccggccac
      961 tgcgtgtgca gccccatgcc tgtggtccag gtgtccttcc tgaccctgcg gaacggcgac
     1021 cagcagggca tccaccacca gccctctggc gtgaaggtgg cccccgagta catgcccagc
     1081 tgcaccgcca gccggcagcg gcggagaatc agacagaccc tgagcggctt cgatatcgcc
     1141 accgtgatgc tggcccagat ggtcaacctg cagagcggca tcagagtgat cagcctgccc
     1201 caggccagcg acatccccac ccctggcacc gacgtgttca tcgtgggcta cggccgggac
     1261 gacaacgacc gggacccatc tagacgggct ggcggcatcc tgaagaaagg cagagccaca
     1321 gtgatggaat gcaagcacag caccaccggc aaccccatct gcgtgcaggc cgcctacgtg
     1381 ttcggccaga tcaccgcccc tggcgactct ggcggccctc tgctgagatc tcctcagggc
     1441 cctgtgctgg gcgtggtgtc tcacggcgtg accctgagca accggctgga cgtgctggtg
     1501 gaatacgcca gcgtggcccg gatgctgggc ttcgtgtcca gcaacattgg cgcgccgtcg
     1561 acctccatca cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac
     1621 cttggagagg aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc
     1681 cagtcctgga tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc
     1741 aaccccaagt tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc
     1801 cttcagtttg ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag
     1861 gaagtgaacc tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag
     1921 gtgatgggac ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc
     1981 agggtctcca ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa
     2041 tgtctactga gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa
     2101 gggttgaatt ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac
     2161 agggacagga acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga
     2221 gtctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat
     2281 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     2341 ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg
     2401 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     2461 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     2521 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     2581 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     2641 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     2701 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     2761 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     2821 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     2881 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     2941 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     3001 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     3061 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     3121 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     3181 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     3241 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     3301 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     3361 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     3421 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     3481 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     3541 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     3601 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     3661 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     3721 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     3781 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     3841 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     3901 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     3961 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     4021 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     4081 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4141 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     4201 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     4261 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4321 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4381 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4441 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4501 agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg
     4561 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4621 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4681 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4741 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4801 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4861 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4921 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4981 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5041 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5101 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5161 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5221 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5281 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5341 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5401 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5461 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5521 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5581 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5641 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5701 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5761 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5821 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5881 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5941 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6001 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6061 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6121 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6181 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6241 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6301 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6361 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6421 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6481 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6541 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6601 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6661 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6781 cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     6841 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
     6901 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
     6961 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     7021 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     7081 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7201 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7261 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     7321 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     7381 acttggcagt acatctacgt attagtcatc gctattacca tg
//
