LOCUS       sequence-234499-        4214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenMOCS backbone for 3 TU in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: pMEG_BB3_YL68N_AD
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(142..161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            761..1880
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        761..1104
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1105..1677
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      1683..1880
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
      721 gcgatcggag accggatccc tcgagggtct ccagctcatg gacatggagg cccagaatac
      781 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      841 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      901 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      961 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     1021 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     1081 catcacatcc gaacataaac aaccatgggt accactcttg acgacacggc ttaccggtac
     1141 cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc
     1201 gacaccgttt ttcgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg
     1261 gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg
     1321 gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg cgacctggcg
     1381 ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga ggacatcgag
     1441 gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt tgatggggct cgcgacggag
     1501 ttcgcccgcg agcggggcgc cgggcacctc tggctggagg tcaccaacgt caacgcaccg
     1561 gcgatccacg cgtaccggcg gatggggttc accctctgcg gcctggacac cgccctgtac
     1621 gacggcaccg cctcggacgg cgagcaggcg ctctacatga gcatgccctg cccctaatca
     1681 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     1741 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     1801 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     1861 gaatgctggt cgctatactg gcttggatcc gaattttcag aacctaattt atctgttacc
     1921 cggcctgtgg ctcgcacagc ttaaaaatgg tcaaactttc cccttcttgt ctttttttcc
     1981 tcacattcat caggttcttg tcttgatctt tcaagtgagt attaattacc gaccttggtt
     2041 cttcattggg agagcattgg aagccgtggt gcagcaacca caaaacggtt cttccccttc
     2101 gataccttct tgcctgcctt tcaatacaag tcggctcgat tagcggtggt cgcccccgcc
     2161 agcggagaac atggaactaa cccagaatga gagctaagtg gagaaagaag agagtcagac
     2221 gactcaagcg aaagcgccgc aaggtccgag ctcgatccaa ataagcggtt tttaacggag
     2281 atttaacact aaatcgaaga acttttcccg tttcatttgc gaatgagctc gttaacaaaa
     2341 tcccccagtt tttttatcca gctgtaagga ttgacattag taatgaatta ttgtttggta
     2401 tatttaaatc tgtagttcct ttctgtccgt gtcggcaact gttgtactcg tgatttactt
     2461 gtattgacga atacttactg tagcgcactc tgctgctact ggtcgtaagg atgtgctatt
     2521 tcggtgtatg gtgggttttt tgggggtcgg aaccgatgac tgttacacgg gcacggctcg
     2581 ttgtgtacac gcacagagct cttgcgagtc atgttgtagc tagctcgtcg tgttcaggaa
     2641 ctgttcgatg gttcggagag agtcgccgcc cagaacatac gcgcaccgat gtcagcagac
     2701 agccttatta caagtacagt atgtacatac tactgtatat tcaagcaagt atatccgtgg
     2761 gtgcgggtga tttggatcta aggttcgtac tcaacactca cgagcagctt gcctatgtta
     2821 catcctttta tcagacataa cataattgga gtttacttac acacggggtg tacctgtatg
     2881 agcaccacct acaattgtag cactggtact tgtacaaaga atttattcgt acgaatcaca
     2941 ggtacggccg ccctcaccga accagcgaat acctcagcgg tcccctgcag tgactcaaca
     3001 aagcgatatg aacatcttgc gatggtatcc tgctgatagt ttttactgta caaacacctg
     3061 tgtagctcct tctagcattt ttaagttatt cacacctcaa ggcgagggat aaattaaata
     3121 aattccaaaa gcgaagatcg agaaactaaa ttaaaattcc aaaaacgaag ttggaacaca
     3181 accccccgaa aaaaaacaac aaacaaaaaa cccaacaaaa taaacaaaaa caaaataaat
     3241 atataactac cagtatctga ctaaaagttc aaatactcgt acttacaaca aatagaaatg
     3301 agccggccaa aattctgcag aaaaaaattt caaacaagta ctggtataat taaattaaaa
     3361 aacacatcaa agtatcataa cgttagttat tttattttat ttaataaaag aaaacaacaa
     3421 aatgggctca aaactttcaa cttatacgat acataccaaa taacaattta gtatttatct
     3481 aagtgctttt cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta
     3541 tacactgaca cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag
     3601 cttgatatat aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta
     3661 agatattaat tatgaactga aagttgatgg catccctaaa tttgatgaaa gatgaaattg
     3721 taaatgaggt ggtaaaagag ctacagtcgt tttgttttga gataccatca tctctaacga
     3781 aatatctatt aaaaatctca gtgtgatcat gagtcattgc caccctggaa aatgtcatca
     3841 tggctgatat ttctaactgt ttacttgaga taaatatata tttacaagaa cttcccttga
     3901 aattaattta gatataaaat gtttgcgggc aagttactac gaggaataaa ttatatctgt
     3961 tgactagaag ttatgaacat tcagtatata tgcacatata ataaccaact tcgaattttc
     4021 caaattagga agtaaattgt tataatgaaa catactttta ggtttttata tattataatg
     4081 gaatacgtct ccattatttt cgccatctaa tttatttaat aaaaaagttt agatagatga
     4141 tctaatttta tatattaaaa tagatggtga gagaaaaaaa aatggcttgc atatatatat
     4201 atatatacag atcc
//
