LOCUS       sequence-234509-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: pMEG_YLCas9_empty
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(142..161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5253..5273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gene            6698..7817
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        6698..7041
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7042..7614
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      7620..7817
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
      721 gcgatcggag agacaccggg ttggcggcgc atttgtgtcc caaaaaacag ccccaattgc
      781 cccaattgac cccaaattga cccagtagcg ggcccaaccc cggcgagagc ccccttctcc
      841 ccacatatca aacctccccc ggttcccaca cttgccgtta agggcgtagg gtactgcagt
      901 ctggaatcta cgcttgttca gactttgttc tagtttcttt gtctggccat ccgggtaacc
      961 catgccggac gcaaaataga ctactgaaaa tttttttgct ttgtggttgg gactttagcc
     1021 aagggtataa aagaccaccg tccccgaatt acctttcctc ttcttttctc tctctccttg
     1081 tcaactcaca cccgaaatcg ttaagcattt ccttctgagt ataagaatca ttcaacatgg
     1141 acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggttgg gccgtcatta
     1201 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
     1261 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca
     1321 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
     1381 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
     1441 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
     1501 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
     1561 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
     1621 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg
     1681 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca
     1741 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc
     1801 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta
     1861 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg
     1921 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga
     1981 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc
     2041 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga
     2101 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc
     2161 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat
     2221 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa
     2281 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac
     2341 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta
     2401 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga
     2461 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat
     2521 tcgcgtggat gactcgcaaa tcagaagaga ctatcactcc ctggaacttc gaggaagtcg
     2581 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc
     2641 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata
     2701 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg
     2761 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga
     2821 aacagctcaa agaggactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg
     2881 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta
     2941 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca
     3001 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc
     3061 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc
     3121 tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt
     3181 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc
     3241 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agccttcacg
     3301 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta
     3361 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg
     3421 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga
     3481 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg
     3541 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca
     3601 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg
     3661 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata
     3721 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt
     3781 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta
     3841 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg
     3901 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca
     3961 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc
     4021 tcgtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc
     4081 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc
     4141 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga
     4201 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata
     4261 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac
     4321 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga
     4381 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga
     4441 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac
     4501 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca
     4561 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg
     4621 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc
     4681 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact
     4741 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga
     4801 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact
     4861 atgaaaagct caaagggtcc cccgaagata atgagcagaa gcagctgttc gtggaacaac
     4921 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc
     4981 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca
     5041 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg
     5101 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg
     5161 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc
     5221 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgagctt
     5281 gcgtctacaa ctggaccctt agcctgtata tatcaattga ttatttaaag atttggtcgg
     5341 taggcggttc gtattgtaca atgggatctg ttactgaggt ggatctaccc aacttgcgag
     5401 attcaattgc gagattcaat cgcgagattc aattgcgaga atcagttgcg agttgttcta
     5461 acactcagct tctacgagcg cttgtattag gacgagtgat actccgtggg gcgacggctt
     5521 ctcttgcgtc ttgtgttgta ttctttctta cactatcgtc catctccaac cacctcgtac
     5581 tcgcacgctc gcctgtactg cttactccaa acaacatgac tggctgacga aatcaaggcg
     5641 ctgtatatat gtacacatct acagcaacag aaggttgttt tgttgcccag ctcgctaata
     5701 gtcttgaaac cgtctccatt cacagctgag acatgtggag gttgcggttc gattgacgct
     5761 ccggggagag acaccgggtt ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc
     5821 caattgaccc caaattgacc cagtagcggg cccaaccccg gcgagagccc ccttctcccc
     5881 acatatcaaa cctcccccgg ttcccacact tgccgttaag ggcgtagggt actgcagtct
     5941 ggaatctacg cttgttcaga ctttgttcta gtttctttgt ctggccatcc gggtaaccca
     6001 tgccggacgc aaaatagact actgaaaatt tttttgcttt gtggttggga ctttagccaa
     6061 gggtataaaa gaccaccgtc cccgaattac ctttcctctt cttttctctc tctccttgtc
     6121 aactcacacc cgaaatcgtt aagcatttcc ttctgagtat aagaatcatt caacatggcg
     6181 tcttcggatc cctcgaggaa gacgcgcttg cgtctacaac tggaccctta gcctgtatat
     6241 atcaattgat tatttaaaga tttggtcggt aggcggttcg tattgtacaa tgggatctgt
     6301 tactgaggtg gatctaccca acttgcgaga ttcaattgcg agattcaatc gcgagattca
     6361 attgcgagaa tcagttgcga gttgttctaa cactcagctt ctacgagcgc ttgtattagg
     6421 acgagtgata ctccgtgggg cgacggcttc tcttgcgtct tgtgttgtat tctttcttac
     6481 actatcgtcc atctccaacc acctcgtact cgcacgctcg cctgtactgc ttactccaaa
     6541 caacatgact ggctgacgaa atcaaggcgc tgtatatatg tacacatcta cagcaacaga
     6601 aggttgtttt gttgcccagc tcgctaatag tcttgaaacc gtctccattc acagctgaga
     6661 catgtggagg ttgcggttcg attgacgcta attcatggac atggaggccc agaataccct
     6721 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     6781 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     6841 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     6901 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     6961 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     7021 cacatccgaa cataaacaac catgggtacc actcttgacg acacggctta ccggtaccgc
     7081 accagtgtcc cgggggacgc cgaggccatc gaggcactgg atgggtcctt caccaccgac
     7141 accgtttttc gcgtcaccgc caccggggac ggcttcaccc tgcgggaggt gccggtggac
     7201 ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg acgaatcgga cgacggggag
     7261 gacggcgacc cggactcccg gacgttcgtc gcgtacgggg acgacggcga cctggcgggc
     7321 ttcgtggtcg tctcgtactc cggctggaac cgccggctga ccgtcgagga catcgaggtc
     7381 gccccggagc accgggggca cggggtcggg cgcgcgttga tggggctcgc gacggagttc
     7441 gcccgcgagc ggggcgccgg gcacctctgg ctggaggtca ccaacgtcaa cgcaccggcg
     7501 atccacgcgt accggcggat ggggttcacc ctctgcggcc tggacaccgc cctgtacgac
     7561 ggcaccgcct cggacggcga gcaggcgctc tacatgagca tgccctgccc ctaatcagta
     7621 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     7681 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     7741 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     7801 tgctggtcgc tatactggct tggatccgaa ttttcagaac ctaatttatc tgttacccgg
     7861 cctgtggctc gcacagctta aaaatggtca aactttcccc ttcttgtctt tttttcctca
     7921 cattcatcag gttcttgtct tgatctttca agtgagtatt aattaccgac cttggttctt
     7981 cattgggaga gcattggaag ccgtggtgca gcaaccacaa aacggttctt ccccttcgat
     8041 accttcttgc ctgcctttca atacaagtcg gctcgattag cggtggtcgc ccccgccagc
     8101 ggagaacatg gaactaaccc agaatgagag ctaagtggag aaagaagaga gtcagacgac
     8161 tcaagcgaaa gcgccgcaag gtccgagctc gatccaaata agcggttttt aacggagatt
     8221 taacactaaa tcgaagaact tttcccgttt catttgcgaa tgagctcgtt aacaaaatcc
     8281 cccagttttt ttatccagct gtaaggattg acattagtaa tgaattattg tttggtatat
     8341 ttaaatctgt agttcctttc tgtccgtgtc ggcaactgtt gtactcgtga tttacttgta
     8401 ttgacgaata cttactgtag cgcactctgc tgctactggt cgtaaggatg tgctatttcg
     8461 gtgtatggtg ggttttttgg gggtcggaac cgatgactgt tacacgggca cggctcgttg
     8521 tgtacacgca cagagctctt gcgagtcatg ttgtagctag ctcgtcgtgt tcaggaactg
     8581 ttcgatggtt cggagagagt cgccgcccag aacatacgcg caccgatgtc agcagacagc
     8641 cttawtacwa nngtayaktm argmaagtat atccgtgggt gcgggtgatt tggatctaag
     8701 gttcgtactc aacactcacg agcagcttgc ctatgttaca tccttttatc agacataaca
     8761 taattggagt ttacttacac acggggtgta cctgtatgag caccacctac aattgtagca
     8821 ctggtacttg tacaaagaat ttattcgtac gaatcacagg aacggccgcc ctcaccgaac
     8881 cagcgaatac ctcagcggtc ccctgcagtg actcaacaaa gcgatatgaa catcttgcga
     8941 tggtatcctg ctgatagttt ttactgtaca aacacctgtg tagctccttc tagcattttt
     9001 aagttattca cacctcaagg cgagggataa attaaataaa ttccaaaagc gaagatcgag
     9061 aaactaaatt aaaattccaa aaacgaagtt ggaacacaac cccccgaaaa aaaacaacaa
     9121 acaaaaaacc caacaaaata aacaaaaaca aaataaatat ataactacca gtatctgact
     9181 aaaagttcaa atactcgtac ttacaacaaa tagaaatgag ccggccaaaa ttctgcagaa
     9241 aaaaatttca aacaagtact ggtataatta aattaaaaaa cacatcaaag tatcataacg
     9301 ttagttattt tattttattt aataaaagaa aacaacaaaa tgggctcaaa actttcaact
     9361 tatacgatac ataccaaata acaatttagt atttatctaa gtgcttttcg tagataatgg
     9421 aatacaaatg gatatccaga gtatacacat ggatagtata cactgacacg acaattctgt
     9481 atctctttat gttaactact gtgaggcgtt aaatagagct tgatatataa aatgttacat
     9541 ttcacagtct gaacttttgc agattaccta atttggtaag atattaatta tgaactgaaa
     9601 gttgatggca tccctaaatt tgatgaaaga tgaaattgta aatgaggtgg taaaagagct
     9661 acagtcgttt tgttttgaga taccatcatc tctaacgaaa tatctattaa aaatctcagt
     9721 gtgatcatga gtcattgcca ccctggaaaa tgtcatcatg gctgatattt ctaactgttt
     9781 acttgagata aatatatatt tacaagaact tcccttgaaa ttaatttaga tataaaatgt
     9841 ttgcgggcaa gttactacga ggaataaatt atatctgttg actagaagtt atgaacattc
     9901 agtatatatg cacatataat aaccaacttc gaattttcca aattaggaag taaattgtta
     9961 taatgaaaca tacttttagg tttttatata ttataatgga atacgtctcc attattttcg
    10021 ccatctaatt tatttaataa aaaagtttag atagatgatc taattttata tattaaaata
    10081 gatggtgaga gaaaaaaaaa tggcttgcat atatatatat atatacagat cc
//
