LOCUS       sequence-234521-        4214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenMOCS backbone for 1 TU in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: pMEG_BB3_YL68N_AB
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2396..2984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2476..2495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            3095..4214
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        3095..3438
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3439..4011
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      4017..4214
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcttggatcc gaattttcag aacctaattt atctgttacc cggcctgtgg ctcgcacagc
       61 ttaaaaatgg tcaaactttc cccttcttgt ctttttttcc tcacattcat caggttcttg
      121 tcttgatctt tcaagtgagt attaattacc gaccttggtt cttcattggg agagcattgg
      181 aagccgtggt gcagcaacca caaaacggtt cttccccttc gataccttct tgcctgcctt
      241 tcaatacaag tcggctcgat tagcggtggt cgcccccgcc agcggagaac atggaactaa
      301 cccagaatga gagctaagtg gagaaagaag agagtcagac gactcaagcg aaagcgccgc
      361 aaggtccgag ctcgatccaa ataagcggtt tttaacggag atttaacact aaatcgaaga
      421 acttttcccg tttcatttgc gaatgagctc gttaacaaaa tcccccagtt tttttatcca
      481 gctgtaagga ttgacattag taatgaatta ttgtttggta tatttaaatc tgtagttcct
      541 ttctgtccgt gtcggcaact gttgtactcg tgatttactt gtattgacga atacttactg
      601 tagcgcactc tgctgctact ggtcgtaagg atgtgctatt tcggtgtatg gtgggttttt
      661 tgggggtcgg aaccgatgac tgttacacgg gcacggctcg ttgtgtacac gcacagagct
      721 cttgcgagtc atgttgtagc tagctcgtcg tgttcaggaa ctgttcgatg gttcggagag
      781 agtcgccgcc cagaacatac gcgcaccgat gtcagcagac agccttatta caagtacagt
      841 atgtacatac tactgtatat tcaagcaagt atatccgtgg gtgcgggtga tttggatcta
      901 aggttcgtac tcaacactca cgagcagctt gcctatgtta catcctttta tcagacataa
      961 cataattgga gtttacttac acacggggtg tacctgtatg agcaccacct acaattgtag
     1021 cactggtact tgtacaaaga atttattcgt acgaatcaca ggtacggccg ccctcaccga
     1081 accagcgaat acctcagcgg tcccctgcag tgactcaaca aagcgatatg aacatcttgc
     1141 gatggtatcc tgctgatagt ttttactgta caaacacctg tgtagctcct tctagcattt
     1201 ttaagttatt cacacctcaa ggcgagggat aaattaaata aattccaaaa gcgaagatcg
     1261 agaaactaaa ttaaaattcc aaaaacgaag ttggaacaca accccccgaa aaaaaacaac
     1321 aaacaaaaaa cccaacaaaa taaacaaaaa caaaataaat atataactac cagtatctga
     1381 ctaaaagttc aaatactcgt acttacaaca aatagaaatg agccggccaa aattctgcag
     1441 aaaaaaattt caaacaagta ctggtataat taaattaaaa aacacatcaa agtatcataa
     1501 cgttagttat tttattttat ttaataaaag aaaacaacaa aatgggctca aaactttcaa
     1561 cttatacgat acataccaaa taacaattta gtatttatct aagtgctttt cgtagataat
     1621 ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca cgacaattct
     1681 gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat aaaatgttac
     1741 atttcacagt ctgaactttt gcagattacc taatttggta agatattaat tatgaactga
     1801 aagttgatgg catccctaaa tttgatgaaa gatgaaattg taaatgaggt ggtaaaagag
     1861 ctacagtcgt tttgttttga gataccatca tctctaacga aatatctatt aaaaatctca
     1921 gtgtgatcat gagtcattgc caccctggaa aatgtcatca tggctgatat ttctaactgt
     1981 ttacttgaga taaatatata tttacaagaa cttcccttga aattaattta gatataaaat
     2041 gtttgcgggc aagttactac gaggaataaa ttatatctgt tgactagaag ttatgaacat
     2101 tcagtatata tgcacatata ataaccaact tcgaattttc caaattagga agtaaattgt
     2161 tataatgaaa catactttta ggtttttata tattataatg gaatacgtct ccattatttt
     2221 cgccatctaa tttatttaat aaaaaagttt agatagatga tctaatttta tatattaaaa
     2281 tagatggtga gagaaaaaaa aatggcttgc atatatatat atatatacag atcccgctgt
     2341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2821 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3001 tttctacggg gtggagcgca aaaaaccccg cccctgacag ggcggggttt tttcgcgatc
     3061 ggagaccgga tccctcgagg gtctccccgg catggacatg gaggcccaga ataccctcct
     3121 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     3181 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     3241 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     3301 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     3361 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     3421 atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc
     3481 agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc
     3541 gtttttcgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg
     3601 cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac
     3661 ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc
     3721 gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc
     3781 ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc
     3841 cgcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc
     3901 cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc
     3961 accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgccccta atcagtactg
     4021 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     4081 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     4141 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     4201 tggtcgctat actg
//
