LOCUS       sequence-234537-        2114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the component plasmid of a unregulated device.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Huang HH, Qian Y, Del Vecchio D
  TITLE     A quasi-integral controller for adaptation of genetic modules to 
            variable ribosome demand.
  JOURNAL   Nat Commun. 2018 Dec 21;9(1):5415. doi: 10.1038/s41467-018-07899-z.
  PUBMED    30575748
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-21"; volume: "9(1):5415. doi"; pages: " 
            10.1038/s41467-018-07899-z"
COMMENT     Sequence Label: pHH04C_Sp_CoD
FEATURES             Location/Qualifiers
     source          1..2114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(771..789)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1187..1978
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      2009..2103
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ccaattattg aacacccttc ggggtgtttt tttgtttctg gtctccccgt tcggctgcgg
       61 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcc
      181 ctcgaggagc gcctcagcgc gccgtagcgt cgataaaaat tacgggctgg ggcgaaacta
      241 ccatctgttc gaaaaggtcc gtaaatgggc ctacagagcg attcgtcagg gctggcctgt
      301 attctcacaa tggcttgatg ccgttatcca gcgtgtcgaa atgtacaacg cttcgcttcc
      361 cgttccgctt tctccggctg aatgtcgggc tattggcaag agcattgcga aatatacaca
      421 caggaaattc tcaccagagg gattttccgc tgtacaggcc gctcgcggtc gcaagggcgg
      481 aactaaatct aagcgcgcag cagttcctac atcagcacgt tcgctgaaac cgtgggaggc
      541 attaggcatc agtcgagcga cgtactaccg aaaattaaaa tgtgacccag acctcgcaaa
      601 atgagaccag ataagcctta tcagataaca gcgccctttt ggcgtctttt tgagcaccta
      661 ggtgtaaaac acggcagtct taagctcggg ccctcttttc tacggggtct gacgctcagt
      721 ggaacgaaaa ctcacgttaa gggattttgg tcatgagtga agacgaaagg gcctcgtgat
      781 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt cggaattgcc
      841 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
      901 gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc
      961 gtttcgcatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1021 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     1081 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1141 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     1201 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1261 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1321 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1381 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     1441 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     1501 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     1561 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     1621 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     1681 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     1741 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     1801 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     1861 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     1921 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataagc
     1981 gggactctgg ggttcgagag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     2041 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     2101 aatccaagca ctag
//
