LOCUS       sequence-234540-        4816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SUC2 promoter with GreenGate A-B flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Lee JE, Neumann M, Duro DI, Schmid M
  TITLE     CRISPR-based tools for targeted transcriptional and epigenetic 
            regulation in plants.
  JOURNAL   PLoS One. 2019 Sep 26;14(9):e0222778. doi: 
            10.1371/journal.pone.0222778. eCollection 2019.
  PUBMED    31557222
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-09-26"; volume: "14(9):e0222778. doi"; pages: " 
            10.1371/journal.pone.0222778. eCollection 2019"
COMMENT     Sequence Label: pSC142
FEATURES             Location/Qualifiers
     source          1..4816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4402..4421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4403..4421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4431..4453)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4647..4666)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4766..4788
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tctaaaatct ggtttcatat taatttcgca
     2281 caccaagtta ctttctatta ttaactgtta taatggacca tgaaatcatt tgcatatgaa
     2341 ctgcaatgat acataatcca ctttgttttg tgggagacat ttaccagatt tcggtaaatt
     2401 ggtattcccc cttttatgtg attggtcatt gatcattgtt agtggccaga catttgaact
     2461 cccgtttttt tgtctataag aattcggaaa catatagtat cctttgaaaa cggagaaaca
     2521 aataacaatg tggacaaact agatataatt tcaacacaag actatgggaa tgattttacc
     2581 cactaattat aatccgatca caaggtttca acgaactagt tttccagata tcaaccaaat
     2641 ttactttgga attaaactaa cttaaaacta attggttgtt cgtaaatggt gctttttttt
     2701 tttgcggatg ttagtaaagg gttttatgta ttttatatta ttagttatct gttttcagtg
     2761 ttatgttgtc tcatccataa agtttatatg ttttttcttt gctctataac ttatatatat
     2821 atatgagttt acagttatat ttatacattt cagatacttg atcggcattt tttttggtaa
     2881 aaaatatatg catgaaaaac tcaagtgttt cttttttaag gaatttttaa atggtgatta
     2941 tatgaatata atcatatgta tatccgtata tatatgtagc cagatagtta attatttggg
     3001 ggatatttga attattaatg ttataatatt ctttcttttg actcgtctgg ttaaattaaa
     3061 gaacaaaaaa aacacatact tttactgttt taaaaggtta aattaacata atttattgat
     3121 tacaagtgtc aagtccatga cattgcatgt aggttcgaga cttcagagat aacggaagag
     3181 atcgataatt gtgatcgtaa catccagata tgtatgttta attttcattt agatgtggat
     3241 cagagaagat aagtcaaact gtcttcataa tttaagacaa cctcttttaa tattttccca
     3301 aaacatgttt tatgtaacta ctttgcttat gtgattgcct gaggatacta ttattctctg
     3361 tctttattct cttcacacca catttaaata gtttaagagc atagaaatta attattttca
     3421 aaaaggtgat tatatgcatg caaaatagca caccatttat gtttatattt tcaaattatt
     3481 taatacattt caatatttca taagtgtgat tttttttttt attgtcaatt tcataagtgt
     3541 gatttgtcat ttgtattaaa caattgtatc gcgcagtaca aataaacagt gggagaggtg
     3601 aaaatgcagt tataaaactg tccaataatt tactaacaca tttaaatatc taaaaagagt
     3661 gtttcaaaaa aaattctttt gaaataagaa aagtgataga tatttttacg ctttcgtctg
     3721 aaaataaaac aataatagtt tattagaaaa atgttatcac cgaaaattat tctagtgcca
     3781 ctcgctcgga tcgaaattcg aaagttatat tctttctctt tacctaatat aaaaatcaca
     3841 agaaaaatca atccgaatat atctatcaac atagtatatg cccttacata ttgtttctga
     3901 cttttctcta tccgaatttc tcgcttcatg gttttttttt aacatattct catttaattt
     3961 tcattactat tatataacta aaagatggaa ataaaataaa gtgtctttga gaatcgaacg
     4021 tccatatcag taagatagtt tgtgtgaagg taaaatctaa aagatttaag ttccaaaaac
     4081 agaaaataat atattacgct aaaaaagaag aaaataatta aatacaaaac agaaaaaaat
     4141 aatatacgac agacacgtgt cacgaagata ccctacgcta tagacacagc tctgttttct
     4201 cttttctatg cctcaaggct ctcttaactt cactgtctcc tcttcggata atcctatcct
     4261 tctcttccta taaatacctc tccactcttc ctcttcctcc accactacaa ccaccgcaac
     4321 aaccaccaaa aaccctctca aagaaatttc ttttttttct tactttcttg gtttgtcaaa
     4381 taacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa
     4441 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4501 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4561 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4621 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4681 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4741 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4801 tcaccgaaac gcgcga
//
