LOCUS       sequence-234571-        7484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized SpCas9 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Wang D, Li J, Song CQ, Tran K, Mou H, Wu PH, Tai PWL, Mendonca CA, 
            Ren L, Wang BY, Su Q, Gessler DJ, Zamore PD, Xue W, Gao G
  TITLE     Cas9-mediated allelic exchange repairs compound heterozygous 
            recessive mutations in mice.
  JOURNAL   Nat Biotechnol. 2018 Oct;36(9):839-842. doi: 10.1038/nbt.4219. Epub 
            2018 Aug 13.
  PUBMED    30102296
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4219"; 
            journalName: "Nat Biotechnol"; date: "2018-10"; volume: "36"; issue:
            "9"; pages: "839-842"
COMMENT     Sequence Label: pAAV.pU1a-SpCas9
FEATURES             Location/Qualifiers
     source          1..7484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      209..664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(296..315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     506..527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(681..700)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     800..822
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(860..878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        946..1050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1051..1119,1120..1911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1269..1288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2082..2670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2571..2590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2732..2861
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             3140..3166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3170..3190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3200..7300
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3200..7300
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7304..7324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7345..7364)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7410..7465
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   join(7478..7484,1..134)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(7478..7484,1..123)
                     /label=AAV2 ITR
ORIGIN
        1 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
       61 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
      121 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      181 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
      241 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
      301 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      361 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      421 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      481 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      541 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
      601 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
      661 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      721 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      781 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      841 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      901 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1081 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1141 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1201 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1261 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1321 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1381 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1441 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1501 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1561 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1621 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1681 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1741 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat
     1801 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1861 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1921 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2701 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     2761 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     2821 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggcctct agaatggagg
     2881 cggtactatg tagatgagaa ttcaggagca aactgggaaa agcaactgct tccaaatatt
     2941 tgtgattttt acagtgtagt tttggaaaaa ctcttagcct accaattctt ctaagtgttt
     3001 taaaatgtgg gagccagtac acatgaagtt atagagtgtt ttaatgaggc ttaaatattt
     3061 accgtaacta tgaaatgcta cgcatatcat gctgttcagg ctccgtggcc acgcaactca
     3121 tactaccggt gccaccatgt acccatacga tgttccagat tacgcttcgc cgaagaaaaa
     3181 gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     3241 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     3301 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     3361 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     3421 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     3481 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     3541 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     3601 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     3661 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     3721 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     3781 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     3841 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     3901 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     3961 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     4021 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     4081 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     4141 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     4201 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     4261 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     4321 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     4381 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     4441 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     4501 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     4561 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     4621 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     4681 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     4741 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     4801 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     4861 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     4921 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     4981 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     5041 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     5101 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     5161 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     5221 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     5281 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     5341 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     5401 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     5461 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     5521 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     5581 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     5641 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     5701 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     5761 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     5821 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     5881 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     5941 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     6001 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     6061 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     6121 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     6181 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     6241 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     6301 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     6361 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     6421 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     6481 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     6541 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     6601 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     6661 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     6721 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     6781 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     6841 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     6901 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     6961 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     7021 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     7081 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     7141 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     7201 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     7261 gtacgagaca cggatcgacc tgtctcagct gggaggcgac agccccaaga agaagagaaa
     7321 ggtggaggcc agctaagaat tcgatctttt tccctctgcc aaaaattatg gggacatcat
     7381 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7441 gtgttggaat tttttgtgtc tctcactcgg cggccgcagg aacc
//
