LOCUS       sequence-23461-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: XE253 pCDNA3.1(zeo)-hDsh3-82-716
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    502..513
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc
      121 ggatccgcca ccatggacta caaggacgac gacgacaagg gcggcgcgcc aggctcacac
      181 ccagacccag cccccttctg tgctgataac ccatcggagc tgccaccacc tatggagcgc
      241 acgggaggca tcggggactc ccgaccccca tccttccacc ctcatgctgg tgggggcagc
      301 caggagaacc tggacaatga cacagagacg gactctttgg tgtctgccca gcgagagcgg
      361 ccacgccgga gggatggccc agagcatgca acccggctaa atggaactgc gaagggggaa
      421 cggcggcgag aaccaggggg ttatgatagc tcatccaccc ttatgagcag tgagctggag
      481 accaccagct tctttgactc agatgaggat gactccacca gcaggttcag cagctccaca
      541 gaacagagca gtgcctcacg cctgatgaga agacacaagc ggcggcggcg gaagcagaag
      601 gtttctcgga ttgagcggtc ctcgtccttc agcagcatca cggactccac catgtcactc
      661 aacatcatca cggtcactct caacatggaa aaatataact tcttgggcat ctccattgtg
      721 gaccaaagca acgagcgtgg tgacggcggc atctacattg gctctatcat gaagggtggg
      781 gcc
//
