LOCUS       sequence-234613-        4901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for Atrial specific Cre recombinase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ni L, Scott L Jr, Campbell HM, Pan X, Alsina KM, Reynolds J, 
            Philippen LE, Hulsurkar M, Lagor WR, Li N, Wehrens XHT
  TITLE     Atrial-Specific Gene Delivery Using an Adeno-Associated Viral 
            Vector.
  JOURNAL   Circ Res. 2019 Jan 18;124(2):256-262. doi: 
            10.1161/CIRCRESAHA.118.313811.
  PUBMED    30582449
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.118"; journalName: "Circ Res"; date: 
            "2019-01-18- 18"; volume: "124"; issue: "2"; pages: "256-262"
COMMENT     Sequence Label: 014LP-pAAV-ANF-657- Cre-pA
FEATURES             Location/Qualifiers
     source          1..4901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     CDS             893..913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1940..1966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1993..2114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2004..2023)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2058..2077
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2262..2391
                     /label=AAV2 ITR
     rep_origin      complement(2628..3216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2708..2727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3387..4178,4179..4247))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4010..4029
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4248..4352)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4378..4833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4515..4536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4727..4746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac atccttaagg
      181 ggccctgtat catgttggct tcctggctga cttcatactc taaaaaaaaa ataatagctc
      241 tttcacctga ctgctaacag ggacatctag tgtgggggtg ggctgtctgg ggccagaggt
      301 ccacccacga ggccgatgaa tcaggtgtga agctagctcc agcatgtgta ctccctggcc
      361 agcctagctg gcctcccagc tgcctgtcat tgcctcctct cccgccctta tttggagccc
      421 ctgacagctg agcagcaagc agggggagct gggtgcaggc cagccgtcac cctctgcttc
      481 cctgcatggg tcctgttgcc agggagaaag aatcctgagg cgagcgccca ggaagataac
      541 caaggactct tttctgctcc tctcacacct ttgaagtggg ggcctcttga ggcaaatcag
      601 caagaatgtg actcttgcag ctgagggtct gggggagggg ggtgagtgga gctgctcaag
      661 gcaaaggggc cgtgacaagc tttgccgaac tgataacttt aaaagggcat cttctgctgg
      721 ctcctcactc catcgcttta tcgctgcaag tgacagaatg gggagggttc tagcccccct
      781 gccttctcaa agagctgggg ggctataaaa acgggagatg ctggcagcta ggagacgaat
      841 tccagaccct ctagaaaata tccaaggatc tggatccaaa ggtaccacca tgcccaagaa
      901 gaagaggaag gtgtccaatc tcctgactgt tcaccagaac ctccctgcgc tgccagtaga
      961 tgccactagc gatgaggtca ggaaaaatct catggatatg tttagggata gacaggcgtt
     1021 ttctgaacac acctggaaaa tgctgcttag cgtgtgccga tcctgggcag cctggtgtaa
     1081 gctgaacaat cgcaaatggt tccccgccga gccggaggac gtgcgcgatt acctgctgta
     1141 tctccaggca agagggctgg ctgtcaagac tatccagcag cacttgggcc aactgaatat
     1201 gctgcatcga cgcagcgggc tcccccggcc tagcgattca aacgcagtct cccttgttat
     1261 gaggagaatt agaaaggaaa acgtagatgc gggtgagagg gctaagcagg ctctcgcttt
     1321 tgagcggact gatttcgacc aggtcagatc cctgatggag aacagcgatc ggtgccagga
     1381 catcaggaac ctcgcatttc tgggaattgc atataacaca cttctgcgca tagctgagat
     1441 cgcccggatc agagtgaaag acatcagtcg aacggacggc ggccggatgc ttattcatat
     1501 tggacgcaca aagacattgg tcagcaccgc tggcgttgaa aaggccttgt ccctgggcgt
     1561 aacgaagctg gtggaaagat ggatctcagt gtccggcgtg gctgacgacc ctaataatta
     1621 cttgttctgt cgagtgagaa aaaacggagt cgccgcgccc tctgccacca gccaattgag
     1681 tacacgggcc cttgaaggga tctttgaggc aacccaccga ctcatatacg gagccaagga
     1741 tgacagtggc cagaggtatc tcgcctggtc aggtcattct gctagggtgg gggccgcacg
     1801 agacatggcg cgggcaggag tctccatacc agagattatg caagctggag gttggacaaa
     1861 tgtgaacatc gttatgaact atatccgcaa tcttgactct gaaaccgggg ccatggtgag
     1921 actgctcgaa gatggtgact acccatacga tgttccagat tacgcttgag cggccgcttc
     1981 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2041 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2101 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     2161 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gagagcatgg
     2221 ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga
     2281 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     2341 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcattaa
     2401 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2461 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2521 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2581 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2641 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2701 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2761 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2821 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2881 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2941 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3001 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3061 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3121 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3181 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3241 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3301 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3361 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3421 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3481 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3541 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3601 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3661 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3721 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3781 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3841 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3901 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3961 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4021 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4081 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4141 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4201 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4261 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4321 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     4381 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4441 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4501 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4561 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4621 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4681 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4741 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4801 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     4861 gttgggaagg gcgatcggtg cgggcctctt cgctattacg c
//
