LOCUS       sequence-23462-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: XE254 pCDNA3.1(zeo)-hDsh3-497-716
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             137..160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttagcagag ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct
      121 cggatccgcc accatggact acaaggacga cgacgacaag ggcggcgcgc cactctgcgg
      181 caacatggcc aacctgtctc tccacgatca cgatggctcc agtggcgcct ctgaccagga
      241 cacactggcc cctttgccgc acccgggggc cgccccttgg cccatggctt tcccgtacca
      301 gtacccgcca cccccgcacc catacaaccc gcacccgggc ttcccggagc tgggctacag
      361 ctacggcggg ggcagcgcca gcagtcagca cagcgaaggc agtcggagca gtggctccaa
      421 ccgtagcggc agcgatcgga ggaaggagaa ggacccgaag gccggggact ccaagtccgg
      481 gggcagcggc agcgaatcgg accacaccac acgcagcagc ctgcgggggc cgcgggagcg
      541 ggcgcccagc gagcgctcag ggccggcggc cagcgagcac agccaccgca gccaccattc
      601 cctggccagc agccttcgca gccaccacac acacccgagc tacntccttc nngggagtgc
      661 ccccttttct acggcccccc catgctgtgg atgcccctgc cgcccgcggc catggggccc
      721 ccaggagccc ctccgggccg cgacctggcc tcagtgcccc cgaantgacc gccagcagac
      781 agtcttncgc atggccatgg aa
//
