LOCUS       sequence-234628-        7050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, 
            Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J
  TITLE     Infection with a Brazilian isolate of Zika virus generates RIG-I 
            stimulatory RNA and the viral NS5 protein blocks type I IFN 
            induction and signaling.
  JOURNAL   Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
  PUBMED    29572905
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483."
COMMENT     Sequence Label: pcDNA3.2/Envelope
FEATURES             Location/Qualifiers
     source          1..7050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    297..321
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1247..1258
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1876..1900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1926..1967
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2007..2026)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2051..2099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2301..2729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2388..2407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2598..2619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2738..2758)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2743..3072
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2923..3058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2985..3004
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3139..3933
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3193..3212)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3803..3822
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4109..4230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4146..4165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4200..4219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4279..4295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4279..4295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4292..4314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4303..4319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4327..4333,4334..4351,4352..4357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4372..4393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4510..4527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4681..5269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4761..4780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5440..6231,6232..6300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6063..6082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6301..6405)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6480..6499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6671..7050
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa
      301 gtttgtacaa aaaagcaggc tccgaattcg cccttaggag gacagctatg atcaggtgca
      361 taggagtcag caatagggac tttgtggaag gtatgtcagg tgggacttgg gttgatgttg
      421 tcttggaaca tggaggttgt gtcaccgtaa tggcacagga caaaccgact gtcgacatag
      481 agctggttac aacaacagtc agcaacatgg cggaggtaag atcctactgc tatgaggcat
      541 caatatcaga catggcttcg gacagccgct gcccaacaca aggtgaagcc taccttgaca
      601 agcaatcaga cactcaatat gtctgcaaaa gaacgttagt ggacagaggc tggggaaatg
      661 gatgtggact ttttggcaaa gggagcctgg tgacatgcgc taagtttgca tgctccaaga
      721 aaatgaccgg gaagagcatc cagccagaga atctggagta ccggataatg ctgtcagttc
      781 atggctccca gcacagtggg atgatcgtta atgacacagg acatgaaact gatgagaata
      841 gagcgaaggt tgagataacg cccaattcac caagagccga agccaccctg gggggttttg
      901 gaagcctagg acttgattgt gaaccgagga caggccttga cttttcagat ttgtattact
      961 tgactatgaa taacaagcac tggttggtcc acaaggagtg gttccacgac attccattac
     1021 cttggcacgc tggggcagac accggaactc cacactggaa caacaaagaa gcactggtag
     1081 agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt tctagggagt caagaaggag
     1141 cagttcacac ggcccttgct ggagctctgg aggctgagat ggatggtgca aagggaaggc
     1201 tgtcctctgg ccacttgaaa tgtcgcctga aaatggataa acttagattg aagggcgtgt
     1261 catactcctt gtgtaccgca gcgttcacat tcaccaagat cccggctgaa acactgcacg
     1321 ggacagtcac agtggaggta cagtacgcag ggacagatgg accttgcaag gttccagctc
     1381 agatggcggt ggacatgcaa actctgaccc cagttgggag gttgataacc gctaaccccg
     1441 taatcactga aagcactgag aactctaaga tgatgctgga acttgatcca ccatttgggg
     1501 actcttacat tgtcatagga gtcggggaga agaagatcac ccaccactgg cacaggagtg
     1561 gcagcaccat tggaaaagca tttgaagcca ctgtgagagg tgccaagaga atggcagtct
     1621 tgggagacac agcctgggac tttggatcag ttggaggcgc tctcaactca ttgggcaagg
     1681 gcatccatca aatttttgga gcagctttca aatcattgtt tggaggaatg tcctggttct
     1741 cacaaatcct cattggaacg ttgctgatgt ggttgggtct gaacacaaag aatggatcta
     1801 tttcccttat gtgcttggcc ttagggggag tgttgatctt cttatccaca gccgtctctg
     1861 ctaagggcga attcgaccca gctttcttgt acaaagtggt tgatctagag ggcccgcggt
     1921 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt
     1981 aatgagttta aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     2041 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     2101 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     2161 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     2221 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg
     2281 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2341 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2401 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2461 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2521 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2581 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2641 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2701 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     2761 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2821 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2881 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2941 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3001 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3061 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     3121 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3181 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3241 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3301 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     3361 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3421 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3481 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3541 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3601 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3661 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3721 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3781 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3841 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3901 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac
     3961 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4021 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4081 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4201 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4261 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6421 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6481 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6541 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     6601 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     6661 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     6721 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     6781 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     6841 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     6901 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     6961 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     7021 atctacgtat tagtcatcgc tattaccatg
//
