LOCUS       sequence-23463-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    784..795
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc
      121 ggatccgcca ccatggacta caaggacgac gacgacaagg gcggcgcgcc tgtacagatt
      181 cgagagggtg gaagtggacc aggtggtatg ggcgagacca agatcatcta ccacttggat
      241 gggcaggaga cgccgtacct tgtgaagctg cccctgcccg ccgagcgcgt caccttggcg
      301 gactttaagg gcgttttgca gcgacccagc tataagttct tcttcaagtc tatggacgac
      361 gatttcggag tggtgaagga ggagatctcg gatgacaatg ccaagctacc atgcttcaat
      421 ggccgggtgg tgtcctggct ggtgtcagct gaggcctcac acccagaccc agcccccttc
      481 tgtgctgata acccatcgga gctgccacca cctatggagc gcacgggagg catcggggac
      541 tcccgacccc catccttcca ccctcatgct ggtgggggca gccaggagaa cctggacaat
      601 gacacagaga cggactcttt ggtgtctgcc cagcgagagc ggccacgccg ganggatggc
      661 ccagagcatg caaccgggct aantggaact gcnaaggtgg gaacggcggc gagaaccagg
      721 gggttatgat agctcatcca cccttatgag cagtgagctg gagacaccag cttctttgac
      781 tcagatgagg atgact
//
