LOCUS       sequence-234630-       10423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10423)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS5
FEATURES             Location/Qualifiers
     source          1..10423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1440..1451)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3342..3366)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3419..3460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3484..4072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3537..3557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3955..3966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4087..4403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4254..4389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4451..4498
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4517..4915
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5002..5235
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5307..5428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5344..5363)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5398..5417
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5468..5603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5623..5642)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5624..5642)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5652..5669)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5652..5668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5661..5683)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5810..6265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5897..5916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6107..6128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6291..6395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6396..6464,6465..7256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6614..6633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7427..8015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7916..7935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8169..8186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8303..8324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8339..8344,8345..8362,8363..8369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8377..8393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8382..8404
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8401..8417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8401..8417
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8436..8456
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8438..8456
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8484..8710
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8711..8891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8938..9063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9556..9789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9974..10018
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10285..10402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggcttcagga ggacagctat ggggggtgga acaggagaga ccctgggaga
      661 gaaatggaag gcccgcttga accagatgtc ggccctggag ttctactcct acaaaaagtc
      721 aggcatcacc gaggtgtgca gagaagaggc ccgccgcgcc ctcaaggatg gtgtggcaac
      781 gggaggccat gctgtgtccc gaggaagtgc aaagctgaga tggttggtgg agcggggata
      841 cctgcagccc tatggaaagg tcattgatct tggatgtggc agagggggct ggagttacta
      901 cgccgccacc atccgcaaag ttcaagaagt gaaaggatac acaaaaggag gccctggtca
      961 tgaagaaccc gtgttggtgc aaagctatgg gtggaacata gtccgtctta agagtggggt
     1021 ggacgtcttt catatggcgg ctgagccgtg tgacacgttg ctgtgtgaca taggtgagtc
     1081 atcatctagt cctgaagtgg aagaagcacg gacgctcaga gtcctctcca tggtggggga
     1141 ttggcttgaa aaaagaccag gagccttttg tataaaagtg ttgtgcccat acaccagcac
     1201 tatgatggaa accctggagc gactgcagcg taggtatggg ggaggactgg tcagagtgcc
     1261 actctcccgc aactctacac atgagatgta ctgggtctct ggagcgaaaa gcaacaccat
     1321 aaaaagtgtg tccaccacga gccagctcct cttggggcgc atggacgggc ctaggaggcc
     1381 agtgaaatat gaggaggatg tgaatctcgg ctctggcacg cgggctgtgg taagctgcgc
     1441 tgaagctccc aacatgaaga tcattggtaa ccgcattgaa aggatccgca gtgagcacgc
     1501 ggaaacgtgg ttctttgacg agaaccaccc atataggaca tgggcttacc atggaagcta
     1561 tgaggccccc acacaagggt cagcgtcctc tctaataaac ggggttgtca ggctcctgtc
     1621 aaaaccctgg gatgtggtga ctggagtcac aggaatagcc atgaccgaca ccacaccgta
     1681 tggtcagcaa agagttttca aggaaaaagt ggacactagg gtgccagacc cccaagaagg
     1741 cactcgtcag gttatgagca tggtctcttc ctggttgtgg aaagagctag gcaaacacaa
     1801 acggccacga gtctgtacca aagaagagtt catcaacaag gttcgtagca atgcagcatt
     1861 aggggcaata tttgaagagg aaaaagagtg gaagactgca gtggaagctg tgaacgatcc
     1921 aaggttctgg gctctagtgg acaaggaaag agagcaccac ctgagaggag agtgccagag
     1981 ttgtgtgtac aacatgatgg gaaaaagaga aaagaaacaa ggggaatttg gaaaggccaa
     2041 gggcagccgc gccatctggt atatgtggct aggggctaga tttctagagt tcgaagccct
     2101 tggattcttg aacgaggatc actggatggg gagagagaac tcaggaggtg gtgttgaagg
     2161 gctgggatta caaagactcg gatatgtcct agaagagatg agtcgcatac caggaggaag
     2221 gatgtatgca gatgacactg ctggctggga cacccgcatc agcaggttcg atctggagaa
     2281 tgaagctcta atcaccaacc aaatggagaa agggcatagg gccttggcat tggccataat
     2341 caagtacaca taccaaaaca aagtggtaaa ggtccttaga ccagctgaaa aagggaaaac
     2401 agttatggac attatttcga gacaagacca aagggggagc ggacaagttg tcacttacgc
     2461 tcttaacaca tttaccaacc tagtggtgca actcattcgg aatatggagg ctgaggaagt
     2521 tctagagatg caagacttgt ggctgctgcg gaggtcagag aaagtgacca actggttgca
     2581 gagcaacgga tgggataggc tcaaacgaat ggcagtcagt ggagatgatt gcgttgtgaa
     2641 gccaattgat gataggtttg cacatgccct caggttcttg aatgatatgg gaaaagttag
     2701 gaaggacaca caagagtgga aaccctcaac tggatgggac aactgggaag aagttccgtt
     2761 ttgctcccac cacttcaaca agctccatct caaggacggg aggtccattg tggttccctg
     2821 ccgccaccaa gatgaactga ttggccgggc ccgcgtctct ccaggggcgg gatggagcat
     2881 ccgggagact gcttgcctag caaaatcata tgcgcaaatg tggcagctcc tttatttcca
     2941 cagaagggac ctccgactga tggccaatgc catttgttca tctgtgccag ttgactgggt
     3001 tccaactggg agaactacct ggtcaatcca tggaaaggga gaatggatga ccactgaaga
     3061 catgcttgtg gtgtggaaca gagtgtggat tgaggagaac gaccacatgg aagacaagac
     3121 cccagttacg aaatggacag acattcccta tttgggaaaa agggaagact tgtggtgtgg
     3181 atctctcata gggcacagac cgcgcaccac ctgggctgag aacattaaaa acacagtcaa
     3241 catggtgcgc aggatcatag gtgatgaaga aaagtacatg gactacctat ccacccaagt
     3301 tcgctacttg ggtgaagaag ggtctacacc tggagtgctg gacccagctt tcttgtacaa
     3361 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     3421 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgatc
     3481 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3541 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3601 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3661 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3721 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3781 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3841 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3901 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3961 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4021 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggcgat
     4081 ggtaccggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4381 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4441 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     4501 ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     4561 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     4621 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     4681 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     4741 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     4801 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     4861 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcaca
     4921 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     4981 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     5041 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5101 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5161 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5221 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5281 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5341 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5401 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5461 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5521 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5581 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5641 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5701 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5761 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5821 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5881 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5941 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6001 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6061 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6121 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6181 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6241 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6481 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6841 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6901 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6961 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7021 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7081 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7141 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7201 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8101 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8161 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8281 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8341 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8401 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8461 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     8521 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8581 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8641 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8701 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8761 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8821 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8881 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     8941 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9001 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9061 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9121 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9181 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9241 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9301 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9361 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     9421 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9481 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9541 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9601 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9661 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9721 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     9781 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     9841 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     9901 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     9961 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10021 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10081 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10141 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10201 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10261 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
    10321 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    10381 aattacaaaa attcaaaatt ttatcgataa gcttgggagt tcc
//
