LOCUS       sequence-234634-        4585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription unit of Y. lipolytica for GoldenMOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: pMEG_BB2_YL001
FEATURES             Location/Qualifiers
     source          1..4585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..73
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2708..2812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2813..2881,2882..3673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3031..3050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3899..4487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4388..4407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
       61 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      121 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      181 caacactagt cgcaaaaaac cccgcccctg acagggcggg gttttttcgc ggtctccgat
      241 cggagagaca ccgggttggc ggcgcatttg tgtcccaaaa aacagcccca attgccccaa
      301 ttgaccccaa attgacccag tagcgggccc aaccccggcg agagccccct tctccccaca
      361 tatcaaacct cccccggttc ccacacttgc cgttaagggc gtagggtact gcagtctgga
      421 atctacgctt gttcagactt tgttctagtt tctttgtctg gccatccggg taacccatgc
      481 cggacgcaaa atagactact gaaaattttt ttgctttgtg gttgggactt tagccaaggg
      541 tataaaagac caccgtcccc gaattacctt tcctcttctt ttctctctct ccttgtcaac
      601 tcacacccga aatcgttaag catttccttc tgagtataag aatcattcaa catgtcatcc
      661 tacgtaggag ctctcgacca gggtaccacc tccacccgtt tcattctctt ttcgcctgac
      721 ggcaagcccg tggcatccca ccagatcgaa ttcacccaga tctaccccca ccccggatgg
      781 gtggagcacg accccgagga gctcgtgagc tcgtgtctgg agtgcatgtc gtcggtggcc
      841 aaggaaatgc gaacccaggg catcaaggtg gccgacgtga aggcgatcgg aatcaccaac
      901 cagcgagaaa ccaccgtgct ttgggacatt gagacgggcc agcccctgta caacgccatt
      961 gtgtggtccg acgcccgaac cggcgacacc gtcaagaagc tcgaggccca gcccggcgct
     1021 gacgaaatcc ccaagctctg tggcctgccc ctgtccacct actttgccgg agtcaaggtc
     1081 cgatggatcc tggataacgt caaggaggcc cgagagtgct acgatcgagg caagctggcc
     1141 ttctccacca tcgactcgtg gctgctctac aacctcacgg gcggcctcaa cggcggcgcc
     1201 cacatcaccg acacctccaa cgcctcccgg tccatgttca tgaacattga gacgctcaag
     1261 tacgacgaga agctcatcaa gttctttggc gtcgagaagc tcattctccc caagattgtc
     1321 tcgtccgccg aggtctacgg ccgaatcgga accggcccct tcgccaacat ccccctggcc
     1381 ggctgtctcg gtgaccagtc cgccgccctc gtcggccaga aggcctttga gcccggccag
     1441 gccaagaaca catatggaac cggctgcttc ctgctctaca acgccggcga gaagcccatc
     1501 atctccaaca acggcctgct gaccaccgtc ggctaccact tcaagggcca gaagcccgtc
     1561 tacgctctgg agggctccat ctccgtcgcc ggctcgtgca tcaagtggct gcgagacaac
     1621 attggtctga ttgagtcatc cgagcagatt ggagagcttg cctcccaggt cgacgactct
     1681 gccggcgtgg tgtttgtcac cgctctgtcc ggcctgtttg ccccctactg gcgaaccgac
     1741 gcccgaggca ccattctggg tctgactcag ttcaccacca aggcccacat ttgccgagcc
     1801 gccctggagg ctacttgttt ccagacccgg gccattctcg acgccatggc caaggactct
     1861 ggtaagccct tcaccaagct gcgagtcgac ggaggaatga ccaactcgga cattgctatg
     1921 cagatccagg ccgacattct tggcattgag gtcgagcgac ccgccatgcg agagacgacc
     1981 gctctgggtg ccgccattgc tgccggcttt gccgttggcg tgtggaagtc cattgaggat
     2041 cttaaggaca tcaacaccga gggcatgacc gagtttgctt ccaagaccaa cgaggaggag
     2101 cgggccgcca tgatgaagca gtggaaccgg ggcattgagc gagctgttgg ctggcttgag
     2161 taagcttgcg tctacaactg gacccttagc ctgtatatat caattgatta tttaaagatt
     2221 tggtcggtag gcggttcgta ttgtacaatg ggatctgtta ctgaggtgga tctacccaac
     2281 ttgcgagatt caattgcgag attcaatcgc gagattcaat tgcgagaatc agttgcgagt
     2341 tgttctaaca ctcagcttct acgagcgctt gtattaggac gagtgatact ccgtggggcg
     2401 acggcttctc ttgcgtcttg tgttgtattc tttcttacac tatcgtccat ctccaaccac
     2461 ctcgtactcg cacgctcgcc tgtactgctt actccaaaca acatgactgg ctgacgaaat
     2521 caaggcgctg tatatatgta cacatctaca gcaacagaag gttgttttgt tgcccagctc
     2581 gctaatagtc ttgaaaccgt ctccattcac agctgagaca tgtggaggtt gcggttcgat
     2641 tgacgctccg gggagaccac cggtgccgct acagggcgcg tcaggtggca cttttcgggg
     2701 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2761 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2821 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2881 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2941 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3001 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3061 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3121 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3181 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3241 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3301 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3361 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3421 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3481 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgcggtat
     3541 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3601 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3661 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3721 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaacaataa
     3781 aactgtctgc ttacataaac agtaatacaa ggggtgtaga tctcatgacc aaaatccctt
     3841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4561 tatcccctga ttctgtggat aaccg
//
