LOCUS       sequence-234637-        3671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenMOCS backbone for 2 TU in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: pMEG_BB3_YL18H_AC
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            5..1580
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        5..348
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             349..1377
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             349..1377
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      1383..1580
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      complement(2977..3565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3057..3076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catggacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
       61 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
      121 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacgtg
      181 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
      241 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
      301 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag
      361 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
      421 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
      481 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
      541 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
      601 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctc
      661 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
      721 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
      781 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
      841 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
      901 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
      961 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     1021 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     1081 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     1141 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     1201 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     1261 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     1321 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca
     1381 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     1441 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     1501 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     1561 gaatgctggt cgctatactg gcttggatcc caatattaca cccaagtagc atgcataagc
     1621 taaaagtaac tcgcagcgca caccgtgcag attcataagt ctatgattaa ttgaacgcca
     1681 ataacccggc ttactacaag tacaagtagg tatacatagc ggtaatgaat cattagaaaa
     1741 aaaaaaaaac aaaaaaaaac aaaacaaact gttgtggatg catcaacagt agtacatagt
     1801 tgtacagtag tacatagttg tacgatgtac ttgtacttgt aaaagcaaaa atgtacaata
     1861 tctcagggag cgcaactttt acgttcgaag aacaatgtac cgcgtaccgc attctggatt
     1921 ctgcggaacg tctaacctgg aaatacgatt ttttttttct ttcatttttt ttgcttcttc
     1981 aaaagtatgg taatttccta ccattacagt tgacactgaa cgagggggga ttgaatttaa
     2041 gcaaaaaatt aaatcaaaat acctttatgt atccagccca tgtaataaac aaaaggatta
     2101 tataacaaga aataaatata tacctttaat ggatcattag aataaaaata ataaatacga
     2161 gaagcacacc agagaagctt tttgattgcc actataccgc tactttggta tattcttatt
     2221 ataattgttg aatttgcaag atagaatgtg ccattcattg gagagaaatc gaaggaatat
     2281 gtgggatgaa atgactagaa gtatgaacaa tgagaatagt acatacttgt acctgtattt
     2341 ctagaagaga gaaagacagt tgagtgtgtg attctcgtcc aataataatc tcaatagtaa
     2401 cgtgtgaata gctgttcttt gatagttgat atttctcgat gactatttat gttgtacaag
     2461 ggattttttt cgttgctgtt gatttcgaat taggcaatgc agatatcatt tatgctatcc
     2521 atatttaaga tttcccatac gcatttataa catttattct acataaattg ttaaatgaac
     2581 gaactgccat tataaattgt ttcctaaata ggaagtgttt ttcataaagc aagtaagttg
     2641 tctaataata ctaagtaata aaaataagtt catacaatat attttgagaa catcatttgg
     2701 aggcggtaga tggagtctgt ttattattaa acaatgcgag atgacccctt aaatattgag
     2761 aacatcagtt ggaggcggca gatggagtct gtctatttag caatgggaca tgactgtcac
     2821 gtatcatcat gatgtatata tataatacat ataatattat ataacacgat ttttttaaat
     2881 tattggcccg aaaattaatc agtgtagact ggatccgctg tgagcaaaag gccagcaaaa
     2941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtggagcgc
     3601 aaaaaacccc gcccctgaca gggcggggtt ttttcgcgat cggagaccgg atccctcgag
     3661 ggtctccaat t
//
