LOCUS       sequence-23464-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..164
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggctttagc agagctctct ggctactaga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg
      121 agctcggatc cgccaccatg gactacaagg acgacgacga caagggcggc gcgcctgtac
      181 agattcgaga gggtggaagt ggaccaggtg gtatgggcga gaccaagatc atctaccact
      241 tggatgggca ggagacgccg taccttgtga agctgcccct gcccgccgag cgcgtcacct
      301 tggcggactt taagggcgtt ttgcagcgac ccagctataa gttcttcttc aagtctatgg
      361 acgacgattt cggagtggtg aaggaggaga tctcggatga caatgccaag ctaccatgct
      421 tcaatggccg ggtggtgtcc tggctggtgt cagctgaggc ctcacaccca gacccagccc
      481 ccttctgtgc tgataaccca tcggagctgc caccacctat ggagcgcacg ggaggcatcg
      541 gggactcccg acccccatcc ttccaccctc atgctggtgg gggcagccag gagaacctgg
      601 acaatgacac agagacggac tctttggtgt ctgcccagcg agagcggcca cgccggaggg
      661 atggcccaga gcatgcaacc cggctaaatg gaactgcgaa gggggaacgg cggcgagaac
      721 cagggggtta tgatagctca tccaccctta tgagcagtga gctggagacc accagcttct
      781 ttgactca
//
