LOCUS       sequence-234640-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS4A-2K-NS4B
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2223..2241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2244..2262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2303..2327
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3573..3597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3650..3691
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3715..4303
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3768..3788)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4186..4197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4318..4634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4485..4620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4682..4729
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4748..5146
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5233..5466
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5538..5659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5575..5594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5629..5648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5699..5834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5854..5873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5855..5873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5883..5900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5883..5899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5892..5914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6041..6496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6128..6147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6338..6359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6522..6626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6627..6695,6696..7487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6845..6864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7658..8246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8147..8166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8400..8417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8534..8555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8570..8575,8576..8593,8594..8600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8608..8624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8613..8635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8632..8648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8632..8648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8667..8687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8669..8687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8715..8941
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgataa gcttgggagt tccgcttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac tagtccagtg
     2281 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctccg aattcgccct
     2341 taggaggaca gctatgggag cggcttttgg agtgatggaa gccctgggaa cactgccagg
     2401 acacatgaca gagagattcc aggaagccat tgacaacctc gctgtgctca tgcgggcaga
     2461 gactggaagc aggccttaca aagccgcggc ggcccaattg ccggagaccc tagagaccat
     2521 tatgcttttg gggttgctgg gaacagtctc gctgggaatc tttttcgtct tgatgaggaa
     2581 caagggcata gggaagatgg gctttggaat ggtgactctt ggggccagcg catggctcat
     2641 gtggctctcg gaaattgagc cagccagaat tgcatgtgtc ctcattgttg tgttcctatt
     2701 gctggtggtg ctcatacctg agccagaaaa gcaaagatct ccccaggaca accaaatggc
     2761 aatcatcatc atggtagcag taggtcttct gggcttgatt accgccaatg aactcggatg
     2821 gttggagaga acaaagagtg acctaagcca tctaatggga aggagagagg agggagcaac
     2881 cataggattc tcaatggaca ttgacctgcg gccagcctca gcttgggcca tctatgctgc
     2941 cttgacaact ttcattaccc cagccgtcca acatgcagtg accacttcat acaacaacta
     3001 ctccttaatg gcgatggcca cgcaagctgg agtgttgttt ggtatgggca aagggatgcc
     3061 attctacgca tgggactttg gagtcccgct gctaatgata ggttgctact cacaattaac
     3121 acccctgacc ctaatagtgg ccatcatttt gctcgtggcg cactacatgt acttgatccc
     3181 agggctgcag gcagcagctg cgcgtgctgc ccagaagaga acggcagctg gcatcatgaa
     3241 gaaccctgtt gtggatggaa tagtggtgac tgacattgac acaatgacaa ttgaccccca
     3301 agtggagaaa aagatgggac aggtgctact catagcagta gcagtctcca gcgccatact
     3361 gtcgcggacc gcctgggggt ggggggaggc tggggccctg atcacagccg caacttccac
     3421 tttgtgggaa ggctctccga acaagtactg gaactcctct acagccactt cactgtgtaa
     3481 catttttagg ggaagttact tggctggagc ttctctaatc tacatagtaa caagaaacgc
     3541 tggcttggtc aagagacgta agggcgaatt cgacccagct ttcttgtaca aagtggttga
     3601 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     3661 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgat cgacaatcaa
     3721 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3781 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3841 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3901 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3961 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4021 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4081 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4141 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4201 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4261 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccggt
     4321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4381 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4441 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4501 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4561 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4621 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     4681 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     4741 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     4801 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     4861 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     4921 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     4981 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     5041 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     5101 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct
     5161 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5221 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5281 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5341 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5401 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5461 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5521 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5641 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5701 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5761 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5821 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5881 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5941 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6001 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6481 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6601 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6661 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6721 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6781 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6841 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6901 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6961 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7021 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7081 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7141 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7201 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7261 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7321 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7381 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7441 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7501 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7561 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7621 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7681 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7741 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8521 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8581 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8641 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8701 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8761 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8821 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8881 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8941 c
//
