LOCUS       sequence-234645-        9677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: hsa-miR 19b plix 4.03
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        223..537
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    231..249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    267..285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    302..320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    338..356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    409..427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    445..463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      554..563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1904..2596
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             2603..2620
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2630..3140
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3059..3077
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3150..3746
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3150..3169)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3646..3666
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3747..3800
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3801..4418,4419..4424,4425..4460,4461..4463,4464..4499,
                     4500..4502,4503..4538,4539..4547)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4587..4607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5005..5016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5221..5454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5526..5647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5563..5582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5617..5636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5687..5822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5822..5893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5894..5962,5963..6754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6112..6131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6925..7513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7414..7433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7667..7684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7801..7822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7837..7842,7843..7860,7861..7867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7880..7902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7899..7915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7899..7915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7934..7954
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7936..7954
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7982..8208
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8209..8389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8436..8561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9054..9287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9472..9516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccgagggga caattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      181 cagggacagc agagatccac tttggccgcg aatcgatatg tcgagtttac tccctatcag
      241 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
      301 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
      421 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
      481 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agaattggct agcgccacca tggatgagga aatcgctgcc ctggtcgttg acaacggctc
      601 cggtatgtgc aaagccggtt ttgctggaga tgatgcccct cgtgctgttt tcccctccat
      661 tgttggacga cccagacatc aggtacattt aataaaccat cctaataaca tgttaatcat
      721 attgaaccac attaaactct catttgtgca ttttacatct tttaaaaccg atttatattc
      781 gtttttagat aatgaagttt attaaagtgt taattcacag ctttattgct tgttttgaga
      841 atctgcttgc ataagtgatt tttaaacttg atctgatttt cgtttgtttc gaagttcttc
      901 agctttgaaa gtcagatctt caattcacag ccatgccata gttttaggat acagtataag
      961 tagacgaatt gggatgcatc gtagatttta tgctgtgttt gttatggttt ttcagtggta
     1021 gagattttca gagtattatt agggttatag tcagtgtcag gtttatttat cgtcagcgtg
     1081 atgggcaaaa aacagctggc agtacgggct gcctgtcgcc caatcaaact gtcctgttac
     1141 tgaacactgt tctatggtta gttttgcagg tttgcatcca gctgtgtgat attctgctgt
     1201 gcaaatccat gcaaaactga ctgtggtagt gaaaagtctg tagaaaagta agggaaactc
     1261 aaaccccttt ctacacaggt tgggatcggt tgcaatgctg tgtttctgta tggtattgca
     1321 cttgtcccgg cctgttgagt ttggtgggga ttgtgaccag aagattttga aaattaaata
     1381 ttactgaaga tttcgacttc cactgttaaa tgtacaagat acatgaaata ttaaagaaaa
     1441 tgtgtaactt tttgtgtaaa tacatcttgt cttgttttca ttcaaaaaca tttcactttt
     1501 ggggttgcgt gagccaactg ctgttactct cgcccagaaa tctgcgccaa catggacaca
     1561 cccagtggag caagccatct gattttcatg gtcagattgt aaagaagcaa ttactcttgg
     1621 acagtttaag cagaaaagcc accaagtcaa gtcactggtc atatgatgaa ctcgaagatc
     1681 taataaaagt taatttaagc gttcattaca ataaattcaa gtgaatctgt tttaagctag
     1741 cctttaacgt ttttaaattc cttcaaacaa attagttcct ctttgcagtt cctcatttct
     1801 cggtgagctg gtgctttaat ccctttactt tttcttcctc tagggagtga tggttggcat
     1861 gggacgaatt aattcctgca gcccgggggt accggtcgcc accatgaaca ccccgggaat
     1921 taacctgatc aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg tgaacggcca
     1981 cgccttcgtg atcgagggcg agggcaaggg caagccctac gagggcaccc agaccgccaa
     2041 cctgaccgtg aaggagggcg cccccctgcc cttcagctac gacatcctga ccaccgccgt
     2101 gcactacggc aaccgggtgt tcaccaagta ccccgaggac atccccgact acttcaagca
     2161 gagcttcccc gagggctaca gctgggagcg caccatgacc ttcgaggaca agggcatctg
     2221 caccatccgc agcgacatca gcctggaggg cgactgcttc ttccagaacg tgcgcttcaa
     2281 gggcaccaac ttccccccca acggccccgt gatgcagaag aaaaccctga agtgggagcc
     2341 cagcaccgag aagctgcacg tgcgcgacgg cctgctggtg ggcaacatca acatggccct
     2401 gctgctggag ggcggcggcc actacctgtg cgacttcaag accacctaca aggccaagaa
     2461 ggtggtgcag ctgcccgacg cccacttcgt ggaccaccgc atcgagatcc tgggcaacga
     2521 cagcgactac aacaaggtga agctgtacga gcacgccgtg gcccgctaca gccccctgcc
     2581 cagccaggtg tggtaaccgg tccaccacca ccaccaccac taaggatccg gggttggggt
     2641 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2701 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     2761 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     2821 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     2881 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     2941 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3001 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3061 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3121 tcaccgacct ctctccccag caattcacca tgaccgagta caagcccacg gtgcgcctcg
     3181 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3241 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3301 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3361 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3421 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3481 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     3541 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3601 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3661 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     3721 ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc tctcctcact tgtggtgatg
     3781 ttgaagaaaa ccctggtcca atgtctagac tggacaagag caaagtcata aacggagctc
     3841 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     3901 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     3961 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     4021 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     4081 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     4141 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     4201 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     4261 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     4321 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     4381 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     4441 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     4501 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc
     4561 gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4621 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4681 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4741 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4801 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4861 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4921 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4981 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5041 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5101 tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag gtacctttaa
     5161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     5461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5821 cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7741 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7801 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7861 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7921 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7981 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8041 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8101 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     8161 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8221 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8281 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8341 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8401 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8461 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8521 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8581 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8641 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8701 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8761 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8821 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8881 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8941 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9001 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9061 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9121 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9181 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9241 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9301 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9361 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9421 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9481 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9541 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9601 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9661 cagacccacc tcccaac
//
