LOCUS       sequence-234651-       13623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drug inducible TALEN targeting the human AAVS1 locus for genome 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13623)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 13623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: 2ERT2-AAVS1 TALEN-L
FEATURES             Location/Qualifiers
     source          1..13623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..341
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1205..1214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1234..1243
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1288..2223
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2284..3219
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5536..6123
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(6316..6336)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6321..6650
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6501..6636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6669..7463
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6723..6742)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7333..7352
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7546..8134
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7599..7619)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8017..8028)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8221..8454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8526..8647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8666..8801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8821..8840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8822..8840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8850..8867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8850..8866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8859..8881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9008..9463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9095..9114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9305..9326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9489..9593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9594..9662,9663..10454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9812..9831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10625..11213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11114..11133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11367..11384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11501..11522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11537..11542,11543..11560,11561..11567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11575..11591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11580..11602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11599..11615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11599..11615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11634..11654
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11636..11654
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11682..11908
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11909..12089
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12136..12261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12754..12987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13172..13216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13483..13600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgaccata tgataagctt tgcaaagatg gataaagttt taaacagaga
       61 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      121 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      181 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      241 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      301 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      361 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      421 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      481 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      661 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
      901 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctcctttc
     1201 tagagccacc atgggttaat tctcctttct agagccacca tgggtggcgc gcctcgcctg
     1261 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca
     1321 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
     1381 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
     1441 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
     1501 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
     1561 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
     1621 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
     1681 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
     1741 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
     1801 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
     1861 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
     1921 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
     1981 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
     2041 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
     2101 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
     2161 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
     2221 acagctttaa ttattggaag tggcgcgcct cgcctgctgg aagatggcga tctcgagcca
     2281 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     2341 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     2401 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     2461 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     2521 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
     2581 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     2641 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     2701 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     2761 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     2821 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     2881 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     2941 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     3001 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     3061 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     3121 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     3181 tattacatca cgggggaggc agagggtttc cctgccacag ctttaattaa tggaagtgga
     3241 gtagatttga gaactttggg atattcacag cagcagcagg aaaagatcaa gcccaaagtg
     3301 aggtcgacag tcgcgcagca tcacgaagcg ctggtgggtc atgggtttac acatgcccac
     3361 atcgtagcct tgtcgcagca ccctgcagcc cttggcacgg tcgccgtcaa gtaccaggac
     3421 atgattgcgg cgttgccgga agccacacat gaggcgatcg tcggtgtggg gaaacagtgg
     3481 agcggagccc gagcgcttga ggccctgttg acggtcgcgg gagagctgag agggcctccc
     3541 cttcagctgg acacgggcca gttgctgaag atcgcgaagc ggggaggagt cacggcggtc
     3601 gaggcggtgc acgcgtggcg caatgcgctc acgggagcac ccctcaacct gaccccagag
     3661 caggtcgtgg caattgcgag caacaagggg ggaaagcagg cactcgaaac cgtccagagg
     3721 ttgctgcctg tgctgtgcca agcgcacgga cttacgccag agcaggtcgt ggcaattgcg
     3781 agcaacggag ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc
     3841 caagcgcacg gactaacccc agagcaggtc gtggcaattg cgagccatga cgggggaaag
     3901 caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cgggttgacc
     3961 ccagagcagg tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc
     4021 cagaggttgc tgcctgtgct gtgccaagcg cacggcttaa ccccagagca ggtcgtggca
     4081 attgcgagcc atgacggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg
     4141 ctgtgccaag cgcacggcct gaccccagag caggtcgtgg caattgcgag ccatgacggg
     4201 ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga
     4261 ctgacaccag agcaggtcgt ggcaattgcg agcaacggag ggggaaagca ggcactcgaa
     4321 accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gactcactcc agagcaggtc
     4381 gtggcaattg cgagccatga cgggggaaag caggcactcg aaaccgtcca gaggttgctg
     4441 cctgtgctgt gccaagcgca cggcctcacc ccagagcagg tcgtggcaat tgcgagccat
     4501 gacgggggaa agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg
     4561 cacggattaa ccccagagca ggtcgtggca attgcgagca acatcggggg aaagcaggca
     4621 ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag cgcacggact taccccagag
     4681 caggtcgtgg caattgcgag ccatgacggg ggaaagcagg cactcgaaac cgtccagagg
     4741 ttgctgcctg tgctgtgcca agcgcacggg ctcaccccag agcaggtcgt ggcaattgcg
     4801 agccatgacg ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc
     4861 caagcgcacg gtcttacccc agagcaggtc gtggcaattg cgagccatga cgggggaaag
     4921 caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cggcctcacc
     4981 ccagagcagg tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc
     5041 cagaggttgc tgcctgtgct gtgccaagcg catgggctca ccccagagca ggtcgtggca
     5101 attgcgagca acatcggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg
     5161 ctgtgccaag cgcacggtct aaccccagag caggtcgtgg caattgcgag ccatgacggg
     5221 ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga
     5281 ctcacgcctg agcaggtagt ggctattgca tccaacatcg ggggcagacc cgcactggag
     5341 tcaatcgtgg cccagctttc gaggccggac cccgcgctgg ccgcactcac taatgatcat
     5401 cttgtagcgc tggcctgcct cggcggacga cccgccttgg atgcggtgaa gaaggggctc
     5461 ccgcacgcgc ctgcattgat taagcggacc aacagaagga ttcccgagag gacatcacat
     5521 cgagtggcag gttcccaact cgtgaagagt gaacttgagg agaaaaagtc ggagctgcgg
     5581 cacaaattga aatacgtacc gcatgaatac atcgaactta tcgaaattgc taggaactcg
     5641 actcaagaca gaatccttga gatgaaggta atggagttct ttatgaaggt ttatggatac
     5701 cgagggaagc atctcggtgg atcacgaaaa cccgacggag caatctatac ggtggggagc
     5761 ccgattgatt acggagtgat cgtcgacacg aaagcctaca gcggtgggta caatcttccc
     5821 atcgggcagg cagatgagat gcaacgttat gtcgaagaaa atcagaccag gaacaaacac
     5881 atcaatccaa atgagtggtg gaaagtgtat ccttcatcag tgaccgagtt taagtttttg
     5941 tttgtctctg ggcatttcaa aggcaactat aaggcccagc tcacacggtt gaatcacatt
     6001 acgaactgca atggtgcggt tttgtccgta gaggaactgc tcattggtgg agaaatgatc
     6061 aaagcgggaa ctctgacact ggaagaagtc agacgcaagt ttaacaatgg cgagatcaat
     6121 ttccgctcat aacctgcagg ggaagtggaa gtgatatcgg aagtggaagc actagtccgg
     6181 awrttgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatggac
     6301 tagtgtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6601 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggat
     6661 ccgccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6721 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6781 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6841 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6901 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6961 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7021 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7081 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7141 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7201 tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7261 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7321 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7381 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7441 gccttcttga cgagttcttc tgaatcgata aaataaaaga ttttatttag tctccagaaa
     7501 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tcgacaatca acctctggat
     7561 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7621 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7681 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7741 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7801 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7861 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7921 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7981 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8041 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8101 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctca gtacctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     8701 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8761 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8821 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9001 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9061 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9361 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9601 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9661 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9721 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9781 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9841 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9901 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9961 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10021 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10081 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10141 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10201 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10261 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10321 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10381 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10441 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10981 cgacctacac caaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11281 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11341 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11401 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    11461 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    11521 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    11581 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    11641 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
    11701 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
    11761 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
    11821 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
    11881 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
    11941 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    12001 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    12061 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    12121 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    12181 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    12241 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    12301 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    12361 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    12421 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    12481 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    12541 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    12601 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    12661 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    12721 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    12781 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    12841 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    12901 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    12961 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    13021 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    13081 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    13141 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    13201 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    13261 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    13321 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    13381 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    13441 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
    13501 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    13561 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
    13621 cga
//
