LOCUS       sequence-234671-       11245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11245)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS5-YFP
FEATURES             Location/Qualifiers
     source          1..11245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1452..1463)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3366..3390)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3443..3445,3446..3958)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(3485..3506)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3746..3765)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4093..4114
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4164..4188)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4241..4282
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4306..4894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4359..4379)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4777..4788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4909..5225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5076..5211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5273..5320
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5339..5737
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5824..6057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6129..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6166..6185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6220..6239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6290..6425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6445..6464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6446..6464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6474..6491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6474..6490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6483..6505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6632..7087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6719..6738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6929..6950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7113..7217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7218..7286,7287..8078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7436..7455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8249..8837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8738..8757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8991..9008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9125..9146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9161..9166,9167..9184,9185..9191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9199..9215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9204..9226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9223..9239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9223..9239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9258..9278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9260..9278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9306..9532
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9533..9713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9760..9885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10378..10611
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10796..10840
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11107..11224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgaat tcgcccttag gaggacagct atggggggtg gaacaggaga
      661 gaccctggga gagaaatgga aggcccgctt gaaccagatg tcggccctgg agttctactc
      721 ctacaaaaag tcaggcatca ccgaggtgtg cagagaagag gcccgccgcg ccctcaagga
      781 tggtgtggca acgggaggcc atgctgtgtc ccgaggaagt gcaaagctga gatggttggt
      841 ggagcgggga tacctgcagc cctatggaaa ggtcattgat cttggatgtg gcagaggggg
      901 ctggagttac tacgccgcca ccatccgcaa agttcaagaa gtgaaaggat acacaaaagg
      961 aggccctggt catgaagaac ccgtgttggt gcaaagctat gggtggaaca tagtccgtct
     1021 taagagtggg gtggacgtct ttcatatggc ggctgagccg tgtgacacgt tgctgtgtga
     1081 cataggtgag tcatcatcta gtcctgaagt ggaagaagca cggacgctca gagtcctctc
     1141 catggtgggg gattggcttg aaaaaagacc aggagccttt tgtataaaag tgttgtgccc
     1201 atacaccagc actatgatgg aaaccctgga gcgactgcag cgtaggtatg ggggaggact
     1261 ggtcagagtg ccactctccc gcaactctac acatgagatg tactgggtct ctggagcgaa
     1321 aagcaacacc ataaaaagtg tgtccaccac gagccagctc ctcttggggc gcatggacgg
     1381 gcctaggagg ccagtgaaat atgaggagga tgtgaatctc ggctctggca cgcgggctgt
     1441 ggtaagctgc gctgaagctc ccaacatgaa gatcattggt aaccgcattg aaaggatccg
     1501 cagtgagcac gcggaaacgt ggttctttga cgagaaccac ccatatagga catgggctta
     1561 ccatggaagc tatgaggccc ccacacaagg gtcagcgtcc tctctaataa acggggttgt
     1621 caggctcctg tcaaaaccct gggatgtggt gactggagtc acaggaatag ccatgaccga
     1681 caccacaccg tatggtcagc aaagagtttt caaggaaaaa gtggacacta gggtgccaga
     1741 cccccaagaa ggcactcgtc aggttatgag catggtctct tcctggttgt ggaaagagct
     1801 aggcaaacac aaacggccac gagtctgtac caaagaagag ttcatcaaca aggttcgtag
     1861 caatgcagca ttaggggcaa tatttgaaga ggaaaaagag tggaagactg cagtggaagc
     1921 tgtgaacgat ccaaggttct gggctctagt ggacaaggaa agagagcacc acctgagagg
     1981 agagtgccag agttgtgtgt acaacatgat gggaaaaaga gaaaagaaac aaggggaatt
     2041 tggaaaggcc aagggcagcc gcgccatctg gtatatgtgg ctaggggcta gatttctaga
     2101 gttcgaagcc cttggattct tgaacgagga tcactggatg gggagagaga actcaggagg
     2161 tggtgttgaa gggctgggat tacaaagact cggatatgtc ctagaagaga tgagtcgcat
     2221 accaggagga aggatgtatg cagatgacac tgctggctgg gacacccgca tcagcaggtt
     2281 cgatctggag aatgaagctc taatcaccaa ccaaatggag aaagggcata gggccttggc
     2341 attggccata atcaagtaca cataccaaaa caaagtggta aaggtcctta gaccagctga
     2401 aaaagggaaa acagttatgg acattatttc gagacaagac caaaggggga gcggacaagt
     2461 tgtcacttac gctcttaaca catttaccaa cctagtggtg caactcattc ggaatatgga
     2521 ggctgaggaa gttctagaga tgcaagactt gtggctgctg cggaggtcag agaaagtgac
     2581 caactggttg cagagcaacg gatgggatag gctcaaacga atggcagtca gtggagatga
     2641 ttgcgttgtg aagccaattg atgataggtt tgcacatgcc ctcaggttct tgaatgatat
     2701 gggaaaagtt aggaaggaca cacaagagtg gaaaccctca actggatggg acaactggga
     2761 agaagttccg ttttgctccc accacttcaa caagctccat ctcaaggacg ggaggtccat
     2821 tgtggttccc tgccgccacc aagatgaact gattggccgg gcccgcgtct ctccaggggc
     2881 gggatggagc atccgggaga ctgcttgcct agcaaaatca tatgcgcaaa tgtggcagct
     2941 cctttatttc cacagaaggg acctccgact gatggccaat gccatttgtt catctgtgcc
     3001 agttgactgg gttccaactg ggagaactac ctggtcaatc catggaaagg gagaatggat
     3061 gaccactgaa gacatgcttg tggtgtggaa cagagtgtgg attgaggaga acgaccacat
     3121 ggaagacaag accccagtta cgaaatggac agacattccc tatttgggaa aaagggaaga
     3181 cttgtggtgt ggatctctca tagggcacag accgcgcacc acctgggctg agaacattaa
     3241 aaacacagtc aacatggtgc gcaggatcat aggtgatgaa gaaaagtaca tggactacct
     3301 atccacccaa gttcgctact tgggtgaaga agggtctaca cctggagtgc tgaagggcga
     3361 attcgaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag
     3421 tctagagggg ccgcggttcg aagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3481 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3541 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3601 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     3661 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3721 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3781 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3841 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3901 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3961 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4021 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4081 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4141 ggacgagctg aagggcgaat tcgacccagc tttcttgtac aaagtggttg atatccagca
     4201 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     4261 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga tcgacaatca acctctggat
     4321 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4381 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4441 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4501 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4561 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4621 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4681 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4741 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4801 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4861 acgagtcgga tctccctttg ggccgcctcc ccgcctggcg atggtaccgg tgtggaaagt
     4921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5041 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5101 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5161 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5221 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     5281 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     5341 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     5401 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     5461 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     5521 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     5581 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     5641 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     5701 tgaattgctg ccctctggtt atgtgtggga gggctaagca caattcgagc tcggtacctt
     5761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5821 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6421 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6601 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7081 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9241 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9301 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     9361 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9421 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     9481 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     9541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9721 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9781 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9841 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9901 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9961 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10021 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10081 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10141 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10201 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10261 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10321 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10381 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10441 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10501 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10561 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10621 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10681 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10741 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10801 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10861 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10921 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10981 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
    11041 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
    11101 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    11161 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    11221 ttttatcgat aagcttggga gttcc
//
