LOCUS       sequence-234698-        4085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4085)
  TITLE     More Bartel Lab Plasmids
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIS1
FEATURES             Location/Qualifiers
     source          1..4085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(545..1133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(625..644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1304..2095,2096..2164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1927..1946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2165..2269)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2402..2523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2439..2458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2493..2512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2594..3529)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(3539..3557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3600..3619)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          3602..3734
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(join(3804..4085,1..470))
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 gtgtacctgt gccccgtcct ggtgtttgtc gcccaacgga cgctccgcgt cagccgcgtg
       61 acccggctcg tcccgcagaa ggtctccggt aatatcaccg cagtcgtgcg gatgctccag
      121 agcctgtcca cgtatacggt ccccatggag cctaggaccc agcgagcccg tcgccgccgc
      181 ggcggcgccg cccgggggtc tgcgagcaga ccgaaaaggt cacactctgg ggcgcgcgac
      241 ccgcccgagt cagcggcccg ccagttacca cccgccgacc aaacccccgc ctccacggag
      301 ggcggggggg tgcttaagag gatcgcggcg ctcttctgcg tgcccgtggc caccaagacc
      361 aaaccccgag ccgcctccga atgagagtgt ttcgttcctt ccccctcccc ccgcgtcaga
      421 caaaccctaa ccaccgctta agcggccccc gcgaggtccg aagactcatt tagatctgtc
      481 gagccatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      601 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      721 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      961 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2281 gaaaagtgcc acctggatcc ttatcgattt taccacattt gtagaggttt tacttgcttt
     2341 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     2401 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2461 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2521 ttatcatgtc tgctcgaagc ggccgctcta gaacactagt agtgatatcg agaccggtat
     2581 agagctccta gaattattgt tcatttttga gaactcgctc aacgaacgat ttgatatatt
     2641 ttcccatttc atcaggtgca tcttcttgcg aaaaatgaag accttttact ttgacaaatt
     2701 cagtattagg aaacttcttg gcaccttcaa caatagcatt ggaaaagaat cctgggtccg
     2761 attcaataaa catttttggt aaatcatcac ttgcacgtag ataagcatta taattcctaa
     2821 caatttgtac aacgtcaggt ttaccacctt ttactaacgg gatttcacga ggccatgata
     2881 atgttggacg acgaacttca cctttctctt tgaatggttc aagatatgct gcaaattctt
     2941 ctggttctaa ctttctcatg atttttgatg gcaacatggt ttccacgaag aagttattct
     3001 ccaaaaccat tttttctcct tcttcagatt tgatcaacgc aatatcttct tcaatatcag
     3061 gccattcatc ccatgattca atcacatcta ctacactttc agcgtgaact attgctttga
     3121 tcttatcttg atgctcatag ctataatgaa atgccaaaca agcaccccaa tcatggccga
     3181 caaaaatgat cttctttggt aaattaagaa gttcaaacca tgcagtaaga tatttgtaat
     3241 gatcaagtaa cctataagaa ccattaccag atttgcctga tttgcccata ccaataaggt
     3301 ctggtataat acaccgcgct actggctcaa tatgtggcac aacatgtcgc cataaataag
     3361 aagaggccgc gttaccatgt aaaaaaataa cagcattttc tgcatgtttt tctgaatcat
     3421 aataattaat aaatgaatca agaacattca tttgtttaca tctggcccac cactgcggac
     3481 cagttatcat ccgtttcctt tgttctggat cataaacttt cgaagtcatg gtggctagcc
     3541 tatagtgagt cgtattaagt actctagcct taagagctgt aattgaactg ggagtggaca
     3601 cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc
     3661 aagagtcttc tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta
     3721 accttgatac ttacctgccc agtgcctcac gaccaacttc tgcagcttaa gttcgagact
     3781 gttgtgtcag aagaatcaag cttttaagcg ggtcgctgca gggtcgctcg gtgttcgagg
     3841 ccacacgcgt caccttaata tgcgaagtgg acctgggacc gcgccgcccc gactgcatct
     3901 gcgtgttcga attcgccaat gacaagacgc tgggcggggt ttgtgtcatc atagaactaa
     3961 agacatgcaa atatatttct tccggggaca ccgccagcaa acgcgagcaa cgggccacgg
     4021 ggatgaagca gctgcgccac tccctgaagc tcctgcagtc cctcgcgcct ccgggtgaca
     4081 agata
//
