LOCUS       sequence-234757-        7863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_026930-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3027..3044
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3096..3151
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3150..3169)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5387..5405)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5473..5577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5578..5646,5647..6438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5796..5815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6609..7197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7098..7117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7351..7368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7485..7506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7521..7526,7527..7544,7545..7551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7559..7575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7564..7586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7583..7599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7583..7599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 cacacatgag caagctgatc agcaccctgc agcagctgag caacagcctg gacgaccaca
     1261 tcaccctgca gatgaagctg cacagcaagc tgaagaaggc caacttccca cccgacaaag
     1321 tgaccatccc cgtgctcgtg atcgcctgca accggcccac cgccagcaga cccatcgaca
     1381 agctgctgca gctgaagcac gagatgctga agcagaacaa cttcgagttc cccatcttcg
     1441 tgtcccacgc ctgcgacgac gacgccaccg agaaggtgct gcacagctac cagaaccgga
     1501 tcaccgtgat caagcccaag acccccctga ccaaccctgt gcagagcagc accagcaagg
     1561 tgttcgaggg ctaccggcac gtgtcccacc actacaagtg ggccctggac cagatcttca
     1621 tggtgtacaa ctacagcacc gtcatcatcg tggaagatga cctggacctg gcccccgact
     1681 tcctgagcta cttcgtgggc gcctacaacc tgctgaccca ggacaacacc ctgttctgcg
     1741 ccagcgcctt caacgacaac ggcagactgc agctgatcga catcaccaga cccgagctgc
     1801 tgtaccggac cgacttcttc cccggcctgg gctggatgct gctgcggaag ttctggctgg
     1861 aaatcagaga gggctggccc gacatctact gggacgagta catgagaaag tcctacgtgc
     1921 ggaagtcccg ggcctgcatc agacccgaga tcagccggtc catcaccttc ggcagagtgg
     1981 gcatcagcca gggccagtac ttcgacagcc acctgaagtt catcaagctg agcagcgcca
     2041 aagtgaactt ccaccagctg gacctgagct acctgcgcga ggacgtgtac cggaaccagt
     2101 tcaagcagct ggtgtacgag gacagcatgg aaatgagctt caaggactac atgaacaacc
     2161 ggcggagcat cccctacaac agcaagagca tccggatcac ctacaagacc cggaaagagt
     2221 tcgaggaaat cgccaagccc ctgggcatca tgctggactt caagtgcggc gtgtcccgga
     2281 acgcctacat gggcgtggtg cccgtgttcg tgaacggcca ccggctgtat atcgcccctc
     2341 ccagcaattg gagcggctac aacgagagct gggtcggcgc gccgtcgacc tccatcacgg
     2401 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     2461 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     2521 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     2581 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     2641 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     2701 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     2761 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     2821 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     2881 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     2941 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     3001 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     3061 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     3121 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3181 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     3241 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     3301 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     3361 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     3421 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     3481 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     3541 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     3601 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     3661 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     3721 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     3781 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     3841 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     3901 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     3961 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     4021 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     4081 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     4141 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     4201 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     4261 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     4321 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     4381 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     4441 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     4501 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     4561 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     4621 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     4681 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     4741 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     4801 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4861 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4921 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4981 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     5041 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     5101 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     5161 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5221 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5281 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     5341 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     5401 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5821 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6121 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7261 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7381 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7441 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7501 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7561 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     7621 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     7681 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     7741 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     7801 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     7861 cgc
//
