LOCUS       sequence-234764-        6864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_194830-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1642..1659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1711..1766
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1765..1784)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4002..4020)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4088..4192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4193..4261,4262..5053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4411..4430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5224..5812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5713..5732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5966..5983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6100..6121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6136..6141,6142..6159,6160..6166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6174..6190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6179..6201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6198..6214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6198..6214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6485..6864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaagcctg gcagagtgaa gtgcagcgcc
      841 cagtgctggg agacaggcat caagtgcaag aacgactgcc ggaacaacgc ctgcaagcgg
      901 aactgccagg acggcgtgcg ggactgcatc cgggaagtgt gcaaggtgtc cgagcacgtg
      961 gacacctacg acgagcacaa gctgcacgat ggcgcgccgt cgacctccat cacggcctat
     1021 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     1081 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     1141 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     1201 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     1261 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     1321 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     1381 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     1441 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     1501 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc
     1561 ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct
     1621 cctctggccc ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa
     1681 ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     1741 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     1801 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
     1861 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     1921 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     1981 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     2041 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     2101 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     2161 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     2221 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     2281 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     2341 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     2401 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     2461 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     2521 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     2581 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     2641 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     2701 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     2761 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     2821 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     2881 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     2941 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     3001 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     3061 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     3121 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     3181 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     3241 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     3301 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     3361 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     3421 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     3481 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     3541 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     3601 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     3661 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     3721 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     3781 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     3841 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     3901 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     3961 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     4021 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4141 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4201 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4261 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4321 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4381 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     4441 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4501 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4561 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4621 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4681 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     4741 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4801 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4861 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4921 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4981 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5041 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5101 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5161 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5221 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5281 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5341 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5401 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5461 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5521 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5581 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5641 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5701 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5761 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5821 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5881 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5941 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6001 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6061 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6121 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6181 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     6241 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     6301 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     6361 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     6421 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     6481 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     6541 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     6601 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     6661 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     6721 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     6781 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     6841 gtattagtca tcgctattac catg
//
