LOCUS       sequence-234765-        7191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_001890-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2349..2366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2418..2473
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2472..2491)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4709..4727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4795..4899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4900..4968,4969..5760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5118..5137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5931..6519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6420..6439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6673..6690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6843..6848,6849..6866,6867..6873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6886..6908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6905..6921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6905..6921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaagaagc
     1201 tgaagctgcc caaagaagtg cgggaagtct ttcagctgca caagtactac cggaacagca
     1261 tccggttctg ccagatgccc aagcagcccc ctgccaagta catgagcaag ctgcagtgga
     1321 acaagcacct ggccgagaag gcccagctga ccgccagcag atgcgactac agctacgaca
     1381 gccccagcga catgagattc gaggaattca gcagcgtggc ccagaatatc gccgactccc
     1441 ccaccatcga gaaggccgtg gccagctggt tcatcgagta caagaactac agcttcgacg
     1501 acaatacctg caaggatacc tgcatgcagt acaagcagat ggtcaagggc gaggaaaccg
     1561 agatcggctg cggcgtgcag aagtgcagca accggttcct ggtcgtgtgc aactacagcc
     1621 ctgccgccga agaggacaag cagccctacg agaagggcac ccaggaaaac tgcgacgacg
     1681 tcgacgacgc cgagtatggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     1741 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     1801 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     1861 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     1921 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     1981 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2041 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2101 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2161 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2221 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     2281 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     2341 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     2401 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2461 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     2521 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2581 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     2641 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     2701 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     2761 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     2821 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     2881 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     2941 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3001 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3061 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3121 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3181 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3241 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3301 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3361 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3421 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3481 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3541 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3601 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     3661 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     3721 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     3781 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     3841 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     3901 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     3961 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4021 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4081 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4141 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4201 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4261 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4321 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4381 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4441 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4501 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4561 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     4681 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4741 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5101 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6901 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     6961 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7021 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     7081 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     7141 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt t
//
