LOCUS       sequence-234771-        6909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_096790-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1687..1704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1756..1811
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1810..1829)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4047..4065)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4133..4237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4238..4306,4307..5098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4456..4475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5269..5857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5758..5777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6011..6028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6145..6166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6181..6186,6187..6204,6205..6211)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6219..6235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6224..6246
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6243..6259
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6243..6259
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6530..6909
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccacacccg aagtgggcga cgagatgctg
      841 ctgaagcgga tggtgctgga caccgccgac aagctgaaga tgctgaccct gaagcacatt
      901 gccgcctgcc ggaacaaggt gcagacctac ttcagagagg acgacctggg cgagaagatc
      961 gccgacgtgc tggtggtgct gctgaaacgg ctgaacaagc ggctggaaaa ctgcctgaag
     1021 tccagcaacc tggaaggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag
     1081 tcagcggagt tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga
     1141 tggaaggcag agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa
     1201 aaggtgtctg tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca
     1261 ctcacccttc agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg
     1321 actctggaca gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag
     1381 cccgacagca acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg
     1441 atcttgaagc aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg
     1501 caggcccctg aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg
     1561 gactccaaga tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg
     1621 gacgcccaga agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg
     1681 ggcccccacc accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga
     1741 cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     1801 tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga
     1861 gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc
     1921 tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa
     1981 ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg
     2041 tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg
     2101 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     2161 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     2221 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     2281 agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc
     2341 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     2401 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc
     2461 tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt
     2521 gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct
     2581 gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt
     2641 gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata
     2701 aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa
     2761 accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag
     2821 aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg
     2881 ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag
     2941 gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga
     3001 gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg
     3061 ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg
     3121 tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa
     3181 gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca
     3241 caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag
     3301 gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca
     3361 cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca
     3421 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     3481 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     3541 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     3601 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     3661 tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg
     3721 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     3781 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     3841 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     3901 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     3961 atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac
     4021 atgagaattt tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4081 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4141 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4201 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4261 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4321 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4381 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4441 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     4501 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4561 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4621 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4681 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4741 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     4801 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4861 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4921 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4981 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5041 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5101 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5161 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5221 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5281 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5341 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5401 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5461 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5521 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5581 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5641 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5701 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5761 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5821 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5881 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5941 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6001 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6061 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6121 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6181 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6241 cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc
     6301 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     6361 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     6421 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     6481 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     6541 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6601 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6661 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6721 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6781 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6841 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6901 attaccatg
//
