LOCUS       sequence-234776-        6972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_141500-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1750..1767
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1819..1874
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1873..1892)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4110..4128)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4196..4300
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4301..4369,4370..5161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4519..4538)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5332..5920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5821..5840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6074..6091
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6208..6229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6244..6249,6250..6267,6268..6274)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6282..6298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6287..6309
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6306..6322
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6306..6322
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6593..6972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaacgagg aagtggacca gcagaaagtg
      841 tgcagcaacc tgaacgagag ctgcagccgg atcctgttcc ggcggtgctg cgacaagctc
      901 gtgtgccagg gcttcttcac cggcacctgt gtggaatgcc tgggcatcaa caagctgtgc
      961 ttcagaagca gcgactgctg cagcggccgg tgctggttct tccactgcaa agagcagaac
     1021 agcaacatca ccaccagcat cgagaacgac accgagacac agcagaacat gaccaacgag
     1081 acactgaccg agacactggg cgcgccgtcg acctccatca cggcctataa gagtgagggg
     1141 gagtcagcgg agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg
     1201 agatggaagg cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac
     1261 caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc
     1321 ccactcaccc ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc
     1381 ctgactctgg acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact
     1441 cagcccgaca gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga
     1501 ctgatcttga agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa
     1561 gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag
     1621 atggactcca agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc
     1681 ctggacgccc agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct
     1741 ctgggccccc accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc
     1801 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1861 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
     1921 ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     1981 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     2041 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     2101 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     2161 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     2221 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     2281 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     2341 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     2401 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     2461 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     2521 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     2581 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     2641 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     2701 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     2761 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     2821 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     2881 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     2941 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     3001 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     3061 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     3121 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     3181 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     3241 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     3301 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     3361 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     3421 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     3481 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     3541 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     3601 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     3661 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     3721 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     3781 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     3841 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     3901 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     3961 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4021 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca
     4081 aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4141 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4201 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4261 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4321 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4381 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4441 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4501 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     4561 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4621 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4681 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4741 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4801 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4861 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4921 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4981 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5041 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5101 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5161 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5221 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5281 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5461 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5881 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5941 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6001 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6061 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6121 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     6181 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6241 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6301 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt
     6361 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     6421 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     6481 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     6541 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     6601 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6661 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6721 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6781 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6841 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6901 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     6961 gctattacca tg
//
