LOCUS       sequence-234777-        7077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_005060-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1855..1872
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1924..1979
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1978..1997)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4215..4233)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4301..4405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4406..4474,4475..5266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4624..4643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5437..6025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5926..5945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6179..6196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6313..6334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6349..6354,6355..6372,6373..6379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6387..6403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6392..6414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6411..6427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6411..6427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6698..7077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgaagtcg tgtgggccgt gaattgtggc
      841 ggccctaagc acatcgacag ccacggcgtc gagtacaagg ccgacttcct ggacatgggc
      901 accgccagcg actacggcag aagcttcgcc atcaaccggg tgcccagcgc cgaccacatc
      961 atctaccaga ccgagagata ccacaacgag gacttcagct accccatccc catcaccagc
     1021 gacggcgagt acatcctgac cctgaagttc agcgaagtgt ggttcaccga gcgctaccag
     1081 aaagtgtcca gcatgatcta cgtgttcagc cactggaccg tgaaacaggt gtcccccgtg
     1141 tactttatcg ccaacatcct gtacagcttc cggctgcacg tgtccgccac cgccaagcac
     1201 attggcgcgc cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc
     1261 tccttcccac tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag
     1321 aaggctcctt cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg
     1381 cagaagtcta ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag
     1441 ataccccagg tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga
     1501 gggatactgt atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac
     1561 actttgacct gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag
     1621 gagaatcagg aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa
     1681 gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc
     1741 caggttttat ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag
     1801 atgctgtgga accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac
     1861 catcaccacc attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg
     1921 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     1981 tcggaaggac atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta
     2041 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
     2101 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
     2161 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
     2221 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
     2281 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
     2341 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
     2401 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
     2461 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
     2521 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
     2581 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     2641 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     2701 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     2761 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     2821 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     2881 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     2941 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     3001 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     3061 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     3121 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     3181 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     3241 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     3301 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     3361 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     3421 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     3481 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     3541 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     3601 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     3661 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     3721 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     3781 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     3841 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     3901 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     3961 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4021 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4081 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4141 aatctgtatc cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc
     4201 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4261 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4381 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4441 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4501 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4561 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4621 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4681 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4741 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4801 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4861 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4921 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     4981 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5041 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5101 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5161 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5221 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5281 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5341 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5821 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6061 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6121 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6181 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6241 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6301 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6361 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6421 ctatgaccat gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc
     6481 ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta
     6541 tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
     6601 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     6661 atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
     6721 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     6781 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6841 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6901 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     6961 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7021 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
