LOCUS       sequence-234780-        6993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_004710-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1188..1197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2151..2168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2220..2275
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2274..2293)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4511..4529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4597..4701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4702..4770,4771..5562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4920..4939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5733..6321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6222..6241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6475..6492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6609..6630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6645..6650,6651..6668,6669..6675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6683..6699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6688..6710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6707..6723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6707..6723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccatggaca
     1201 gcgacaacga ccccagcgac atcagcgccg acaagcggtt cctgctggcc ctgcccagcc
     1261 ccaagagact gagccagccc agctacagct tcaaccggta cagacggcct atgtacggct
     1321 acggctaccg gcccgactac atcgacgccg acgacgacct gttcaacgag gacaagagat
     1381 ttctgctggg cctgcctccc aaggtggaac acaaacggtt tctgctcgga ctgcccccca
     1441 gcctgcggca gcacaagcgg tttatcctgg gactgcctgc ccccacccgg ttccactctg
     1501 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct
     1561 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg
     1621 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga
     1681 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac
     1741 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga
     1801 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt
     1861 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga
     1921 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag
     1981 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg
     2041 ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc cagaagatgc
     2101 tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc caccaccatc
     2161 accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     2221 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2281 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     2341 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     2401 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     2461 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     2521 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     2581 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     2641 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     2701 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     2761 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     2821 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     2881 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     2941 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     3001 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     3061 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     3121 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     3181 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     3241 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     3301 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     3361 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     3421 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     3481 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     3541 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     3601 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     3661 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     3721 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     3781 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     3841 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     3901 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     3961 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4021 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4081 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4141 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     4201 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4261 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4321 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     4381 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     4441 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     4501 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4561 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4621 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4681 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4741 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4801 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4861 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4921 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     4981 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5041 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5101 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5161 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5221 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     5281 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5341 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5401 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5461 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5521 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5581 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5641 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5701 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5761 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5821 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5881 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5941 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6001 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6061 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6601 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6661 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6721 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     6781 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
     6841 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
     6901 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
     6961 atattggctc atgtccaata tgaccgccat gtt
//
