LOCUS       sequence-234782-        7401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_123550-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2559..2576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2628..2683
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2682..2701)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4919..4937)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5005..5109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5110..5178,5179..5970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5328..5347)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6141..6729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6630..6649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6883..6900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7017..7038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7053..7058,7059..7076,7077..7083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7096..7118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7115..7131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7115..7131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gcccagccta
     1201 aggacgccca gctgagaaag ctgctgcagt accacaacga gctgcggcgg aacctgaccg
     1261 cctgcaagct ggaaggccag ccccctgcca agaacctgct ggacctgaag tgggacaatg
     1321 agctggccag caaggccaag gacctggcca acgagtgcta cttccaccac aacgacgtgg
     1381 acctgcccga gaagtggcag tacatcggcc agaatatcgc cggctaccag accatcgagc
     1441 aggccttcga cgcctggaag gacgagtaca agcagtacaa ctactacagc aagagctgca
     1501 gcggcgtgtg cggccactac acccagctcg tgtggcagaa caccacccac gtgggctgcg
     1561 gcatcaccaa ctgtaccggc agctacagct tcccctacgg cctgagcgtc gtgtgcaact
     1621 acggccctgg cggcaactac gagggcagat acccctacga ggccaagagc caggatgagt
     1681 gctacgccac caccaccaag cggcccacca ccacaaaaag acccacaacc actaagaggc
     1741 ccagcacaac taccaccaag aggcctggca ccacccccac ccagaaaccc ggcgtgccca
     1801 agcagatccc caagcccatc tggcccagca tcatcagcac ctggaacgag tacgccacca
     1861 gcaacatgat ccagggcatc gtgacccaga cctgcatctg cgtgaaaggc gcgccgtcga
     1921 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     1981 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     2041 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     2101 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     2161 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     2221 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     2281 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     2341 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     2401 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     2461 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     2521 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     2581 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2641 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2701 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     2761 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     2821 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     2881 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     2941 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3001 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3061 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3121 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     3181 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3241 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3301 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     3361 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3421 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3481 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3541 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3601 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3661 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     3721 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     3781 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     3841 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     3901 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     3961 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4021 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4081 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4141 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     4201 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4261 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4321 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4381 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4441 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4501 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4561 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4621 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4741 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     4801 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     4861 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     4921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5341 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5641 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7021 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7081 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7141 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     7201 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     7261 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     7321 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     7381 gtccaatatg accgccatgt t
//
