LOCUS       sequence-234788-        9511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Chong-Morrison V, Mayes S, Simoes FC, Senanayake U, Carroll DS, 
            Riley PR, Wilson SW, Sauka-Spengler T
  TITLE     Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in 
            zebrafish.
  JOURNAL   Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. 
            Epub 2023 Jun 27.
  PUBMED    37367831
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2023-06-15"; volume: "12(6):bio059995. doi"; pages: "
            10.1242/bio.059995. Epub 2023 Jun 27"
COMMENT     Sequence Label: pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
FEATURES             Location/Qualifiers
     source          1..9511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     350..369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(588..692)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(718..1173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(855..876)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1067..1086
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1300..1322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1314..1331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1315..1331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1341..1360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1341..1359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5137..5146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5150..5536
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5591..5611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5627..6169
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             6194..6565
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             6581..6634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6647..6649,6650..7363)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6689..6710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6950..6969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7297..7318
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7415..7536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7426..7445)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7480..7499
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8038..8058)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8038..8056)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8077..8093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8077..8093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8090..8112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8101..8117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8125..8131,8132..8149,8150..8155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8170..8191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8308..8325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8479..9067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8559..8578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9238..9511,1..518,519..587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
       61 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
      721 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      781 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      841 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      901 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      961 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     1021 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     1081 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     1141 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     1201 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     1261 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     1321 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acccgacgca
     1381 gggatgaaag taggatggga aaatcccgta ccgaccgtta tcgtataacc gattttgtta
     1441 gttttatccc gatcgatttc gaacccgagg taaaaaacga aaacggaacg gaaacgggat
     1501 atacaaaacg gtaaacggaa acggaaacgg tagagctagt ttcccgaccg tttcaccggg
     1561 atcccgtttt taatcgggat gatcccgttt cgttaccgta ttttctaatt cgggatgact
     1621 gcaacagatc cctcgactcg agaccagcaa agttctagaa tttgtcgaaa catttatgtt
     1681 atatatttcc tgaaaaaaat tctgagtaag ttcttaagtg ttattgccag caacataaac
     1741 aacagacggc aaaatgaata aatgataaca aagcagtagg cttaaataaa cctaattttt
     1801 ataggctgtt ctctacaacc ctcaaacagt gattagtttt gtacttataa acttgccctt
     1861 tcattcatat ttcaagaaaa ttggttcaga agatctggat attctagcag ttgttcaagc
     1921 tcatggaggg atcagtgacc tgattccaca atgactaggc ctaatccaga aattagatga
     1981 ctgtcaacat aaaaaggcac agcactcact agctgcccta tatattttat tatattttac
     2041 atatattatt ttatttattt agctctgagt gctgtacttt ctggttaaag aaaactgctt
     2101 acaacagcta acctgtacta cctcaggctc agggaatttg gaacaggttt gtctggtttg
     2161 tttctttaac catgcatgct tgttttcaac tatggcaaca cagtcacatg ggacattaca
     2221 gaaatgattt gtcgatgaca tgcgactttt ctttaataaa gcgcaaagat cccaaaaagc
     2281 aaacttttaa caaaaatcat ataattatat tttcaatcca gctttgtagc aactttgtgc
     2341 tgctgttcac tcagcaacag atagtcagta taaggtcagt gtgtctcaaa gcagtgccat
     2401 ctgtttcaca cattgcgttc tatatataag tgtgctggtt gacacgacac tgtataaggc
     2461 ctaggctaaa acacaaacaa tgtagaatga cactgtgttt tttttgtaaa caaatgttgt
     2521 ttttggttaa acatctttgt gaaaacatcc tcctgtcatg tatttgctat attcaaatgt
     2581 taaacccgtg cagaatagaa catatacaaa aaaaaacaac acaacacatt tttaaacatt
     2641 attaaatatc aagtattgct ggcagttctg tttctgtttt acagtaccct ttgccacagt
     2701 tctccgcttt tcctggtcca gattccacaa gtctgattca ccaatagcaa agcgaataaa
     2761 caaccaaagc agccaatcac tgcttgtaga ctgtcctgcg agaccggccc attccagcac
     2821 attctggaaa cttcctttat atgataatta taaatacatt taaattattg atacaaaaca
     2881 tgtaattcct agaacataac catagcaatc attagttttc agggtaatta tgtattttta
     2941 ggatttgact gcggaaagat ctggtcatgt gacgtctcat gaacgtcacg gccctgggtt
     3001 tctataaata cagtaggact ctcgaccatc ggcagatttt tcgaagaaga agatcagttt
     3061 caggagccgt actgttccgt ttcaacgcaa atattaacgg taagagcgaa tttcctagtt
     3121 tgttttcatg ccattcttta aaaccatagc gtattacttt aattatagta aactttcgct
     3181 ttctttatta caagagacgt tttgtgttga ttctccgcgg acattttcgg tcagacaatc
     3241 agaaaatgac cgcggaggac cagtaacttg cattacacgt aagttaaatc ttcgtgtatt
     3301 aaaatggtta ggttgttaac gtcaaatagg ttaccgtgtt tgcgtgtgat caggttggtt
     3361 ttgttagatt tttgtcagta tttttaattt atttgtttta gtttatttat tttttttgct
     3421 gaatcatagt ttgtgaacaa agaacccgga tgttacatac agtacagccg ccatgttaca
     3481 gagagttata acttaatcat tttaaaaata attttgcctt acttttagtt tgtcatgttg
     3541 agaaatgagg aaatgttaaa atgaggaaat atccaattaa tttaatatat caaaataatc
     3601 catgattaca atgcactgaa ctggagaaaa ttaagatgtt ttctagtgtc atgaaacaaa
     3661 tgtaagagat gtacattgta gatgttttat gtcaagaatt ggctagttga tgcagcatac
     3721 tggcgatact cagttgtaat aacagtaacg ttacatgtta atagactact gagtatgctg
     3781 ttctgtctat gtatgctctg taagctacga gaaggacttt tttaaacagt aaagggtgca
     3841 atatttttac aaattgaatt aaataaaggc tgtctattaa gtaatatgct tgatattttt
     3901 cttacttgat cggaaataag aaaaaatata aacgttgttg ctctaaaaat cctagttcag
     3961 tttagccaac cacaaatacc tttttgttcc tccaacagtt tttttttctt ctctataata
     4021 tttggcagtc tatagtactc caaatgtttc cccacagtct aactaattgg tacagccaaa
     4081 atcatgacac ttattgcaat aataattttg gttcattggc attgttgata gcctgtgcca
     4141 ctaatatggt cgattgatca tgcttcagga agaaaactat attgtttgat gtaagattat
     4201 taaatcttca cctgcttcca ttacaaacta ttcccatctt attgaattct ggtatgtctt
     4261 aaaggattag ttcacttccc aaatcaaaat ttacttagcc ttttttcatc catgatccct
     4321 ttttttcatc attaatgaag aaattgtttt tgaaaaagtt tcaagatttt tttctctata
     4381 ttgtggagct tgttagttta aaattccaaa atgcaatatg tggcttcaaa tggttctaaa
     4441 tgatcccagt caaggaataa cagtcttatc taatgaaacc attagacctt taaaaataaa
     4501 aaaaataaaa gtatttattt ttaaatgact gagtgattaa gttgaatttc agcgtttcct
     4561 tactgtgtag aagtccttcc ttactggccc caccctttgg ttctctgcca atctgctacc
     4621 taatgtaatg ttgtggaaca ttattattct ttatttctta attttttatt ttttatttta
     4681 aaacaatgta aactgcacag atgtgcagtt tgtttaaaat ggccaatgct ttggaaatgc
     4741 atgacataat tagatttcat gatgcacaaa gccaaatctc agagcttgtg caaaatgagc
     4801 tatcatttca ctaggtaaga ccctaaattt tcatatagga tcatttggac aattttgctg
     4861 caggtaaaat gcattctata gtccactgtc agccattgtt ttggatagta tttatttttc
     4921 tctacaagta tagtcaatag ttttctatta ttttaaaggt ttgtaacatt taagggtgac
     4981 caaatgcaaa gtaaaatttc attttcgggt gaactatctc gtttaacatg ggagaagtgc
     5041 aaaacataca ttattggcta gaacattgta gtatttttta aatggaaatg tgtgattgct
     5101 aatcttactt tgaatttgtt tacaggtacc gctagcgcca ccatggatgg cttcaaactt
     5161 tactcagttc gtgctcgtgg acaatggtgg gacaggggat gtgacagtgg ctccttctaa
     5221 tttcgctaat ggggtggcag agtggatcag ctccaactca cggagccagg cctacaaggt
     5281 gacatgcagc gtcaggcagt ctagtgccca gaagagaaag tataccatca aggtggaggt
     5341 ccccaaagtg gctacccaga cagtgggcgg agtcgaactg cctgtcgccg cttggaggtc
     5401 ctacctgaac atggagctca ctatcccaat tttcgctacc aattctgact gtgaactcat
     5461 cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct atcccttccg ccatcgccgc
     5521 taactcaggt atctacagcg ctggaggagg tggaagcgga ggaggaggaa gcggaggagg
     5581 aggtagcgga cctaagaaaa agaggaaggt ggcggccgct ggatcccctt cagggcagat
     5641 cagcaaccag gccctggctc tggcccctag ctccgctcca gtgctggccc agactatggt
     5701 gccctctagt gctatggtgc ctctggccca gccacctgct ccagcccctg tgctgacccc
     5761 aggaccaccc cagtcactga gcgctccagt gcccaagtct acacaggccg gcgaggggac
     5821 tctgagtgaa gctctgctgc acctgcagtt cgacgctgat gaggacctgg gagctctgct
     5881 ggggaacagc accgatcccg gagtgttcac agatctggcc tccgtggaca actctgagtt
     5941 tcagcagctg ctgaatcagg gcgtgtccat gtctcatagt acagccgaac caatgctgat
     6001 ggagtacccc gaagccatta cccggctggt gaccggcagc cagcggcccc ccgaccccgc
     6061 tccaactccc ctgggaacca gcggcctgcc taatgggctg tccggagatg aagacttctc
     6121 aagcatcgct gatatggact ttagtgccct gctgtcacag atttcctcta gtgggcaggg
     6181 aggaggtgga agcggcttca gcgtggacac cagtgccctg ctggacctgt tcagcccctc
     6241 ggtgaccgtg cccgacatga gcctgcctga ccttgacagc agcctggcca gtatccaaga
     6301 gctcctgtct ccccaggagc cccccaggcc tcccgaggca gagaacagca gcccggattc
     6361 agggaagcag ctggtgcact acacagcgca gccgctgttc ctgctggacc ccggctccgt
     6421 ggacaccggg agcaacgacc tgccggtgct gtttgagctg ggagagggct cctacttctc
     6481 cgaaggggac ggcttcgccg aggaccccac catctccctg ctgacaggct cggagcctcc
     6541 caaagccaag gaccccactg tctccgatat cggatcagga gagggcaggg gaagtcttct
     6601 aacatgcggg gacgtggagg aaaatcccgg gccccggtcg cgatcggtga gcaagggcga
     6661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6781 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6841 ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaaag acgacggcaa
     6961 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7021 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc
     7081 catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     7141 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7201 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7261 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     7321 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     7381 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     7441 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7501 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     7561 atgtttcggg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     7621 tgtggtacca tggtctagaa gtagacttat atggcttctt atgttagcca agagcccaag
     7681 acttatcact tatgtgctac attaaactat gtgtgctcca gatttatatg gattttatct
     7741 atgtttaatt aagacttgtg tttacaattt tttatatttg tttttaagtt ttgaatatat
     7801 gttttcatgt gtgattttac cgaacaaaaa taccggttcc cgtccgattt cgactttaac
     7861 ccgaccggat cgtatcggtt ttcgattacc gtatttatcc cgttcgtttt cgttaccggt
     7921 atatcccgtt ttcgtttccg tcccgcaagt taaatatgaa aatgaaaacg gtagaggtat
     7981 tttaccgacc gttaccgacc gttttcatcc ctagtcgggg agctccagct tttgttccct
     8041 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8101 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8161 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8221 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8281 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8341 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8401 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8881 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9121 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9181 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9241 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9301 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9361 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9421 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9481 tattaattgt tgccgggaag ctagagtaag t
//
