LOCUS       sequence-234802-       12213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NOTCH2NLB full length under CAG promoter 
            with mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 12213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry
FEATURES             Location/Qualifiers
     source          1..12213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2907..3184
                     /label=chicken beta-actin promoter
     intron          3185..4197
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4121..4143
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4205..4224
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4260..4279
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4260..4278
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4293..4311
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4293..4310
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5169..5186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5211..5240
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5250..5279
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5289..5318
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5328..5357
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5367..5396
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5430..5459
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(5514..6088,6089..6091,6092..6100)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5681..5698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5908..5927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6132..6842
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6277..6295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6545..6564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6902..7490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6955..6975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7373..7384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7493..7509)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8018..8198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8224..8241)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8230..8454
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8500..8928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8587..8606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8797..8818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8937..8957)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8942..9271
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9122..9257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9184..9203
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9319..9366
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9385..9759
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9889..10010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9926..9945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9980..9999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10059..10075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10059..10075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10072..10094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10083..10099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10107..10113,10114..10131,10132..10137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10152..10173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10290..10307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10461..11049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10541..10560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11220..12011,12012..12080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11843..11862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12081..12185)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     3061 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3181 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
     3241 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     3301 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
     3361 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
     3421 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
     3481 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     3541 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     3601 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     3661 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     3721 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     3781 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     3841 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     3901 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     3961 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     4021 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     4081 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     4141 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     4201 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagcgt
     4261 aatacgactc actataggga gacccaagct tgatttaggt gacactatag aatacaagct
     4321 acttgttctt tttgcagacc accatgcccg ccctgcgccc cgctctgctg tgggcgctgc
     4381 tggcgctctg gctgtgctgc gcgacccccg cgcatgcatt gcagtgtcga gatggctatg
     4441 aaccctgtgt aaatgaagga atgtgtgtta cctaccacaa tggcacagga tactgcaaat
     4501 gtccagaagg cttcttgggg gaatattgtc aacatcgaga cccctgtgag aagaaccgct
     4561 gccagaatgg tgggacttgt gtggcccagg ccatgctggg gaaagccacg tgccgatgtg
     4621 cctcagggtt tacaggagag gactgccaat actcgacatc tcatccatgc tttgtgtctc
     4681 gaccttgcct gaatggcggc acatgccata tgctcagccg ggatacctat gagtgcacct
     4741 gtcaagtcgg gtttacaggt aaggagtgcc aatggaccga tgcctgcctg tctcatccct
     4801 gtgcaaatgg aagtacctgt accactgtgg ccaaccagtt ctcctgcaaa tgcctcacag
     4861 gcttcacagg gcagaagtgt gagactgatg tcaatgagtg tgacattcca ggacactgcc
     4921 agcatggtgg catctgcctc aacctgcctg gttcctacca gtgccagtgc cttcagggct
     4981 tcacaggcca gtactgtgac agcctgtatg tgccctgtgc accctcgcct tgtgtcaatg
     5041 gaggcacctg tcggcagact ggtgacttca cttttgagtg caactgcctt ccagaaacag
     5101 tgagaagagg aacagagctc tgggaaagag acagggaagt ctggaatgga aaagaacacg
     5161 atgagaatca ccaccatcat caccacggat cccatcgatt taaagctatg gagcaaaagc
     5221 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga
     5281 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
     5341 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
     5401 aaatggagag cttgggcgac ctcaccatgg agcaaaagct catttctgaa gaggacttga
     5461 atttagagat ctagaattcg atgtgtgtta cctacgctag cgttaacgat ccgcccctct
     5521 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5581 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5641 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5701 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5761 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5821 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5881 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5941 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc
     6001 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6061 gttttccttt gaaaaacacg atgataatat ggccacaacc ggctggagac gtggaggaga
     6121 accctggacc tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     6181 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     6241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     6301 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6361 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     6421 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     6481 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     6541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     6601 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     6661 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     6721 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     6781 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     6841 aagcggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     6901 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7021 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7081 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7141 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7201 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7261 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7321 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7381 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7441 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     7501 tcgacctcgt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7561 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7621 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7681 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7741 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     7801 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     7861 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     7921 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     7981 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     8041 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8101 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8161 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     8221 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8281 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8341 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8401 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8461 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     8521 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8581 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8641 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8701 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8761 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8821 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8881 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     8941 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9001 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9061 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9181 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     9241 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     9301 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     9361 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     9421 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9481 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9541 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     9601 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9661 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9721 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     9781 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9841 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    10021 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10141 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10201 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12181 ccgcgcacat ttccccgaaa agtgccacct gac
//
