LOCUS       sequence-23482-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang
            X, Civin CI, Cheng L
  TITLE     Lentiviral vectors with two independent internal promoters transfer 
            high-level expression of multiple transgenes to human hematopoietic 
            stem-progenitor cells.
  JOURNAL   Mol Ther. 2003 Jun . 7(6):827-38.
  PUBMED    12788657
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2003 Jun . 7(6):827-38."
COMMENT     Sequence Label: EF.PGK.GFP
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        113..623
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     542..560
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      639..648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(690..711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg taagcttgat
       61 atcgaattcc tgcagcccgg gggatctcga gaagcttgat atcgaattcc acggggttgg
      121 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
      181 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
      241 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
      301 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
      361 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
      421 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
      481 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
      541 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
      601 gaatcaccga cctctctccc cagggggatc caccggtcgc caccatggtg agcaagggcg
      661 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      721 acaagt
//
