LOCUS       sequence-234841-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pheromone responsive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS053
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(537..571)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(642..1301)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1216..1235
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1483..2071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1972..1991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacggaact ggttgatatt attactggtg cttcctcttt gggatgataa
       61 aaaaatcacc ctgcattgcc tctttatttg acgttgtttt tgtagaacat gaaacgaatt
      121 ttgacttgat gacacgaagt atatatccaa agaatacctt aaatagaaaa ggaaagataa
      181 taaatactaa acactactat atattcaggt aaaatacaaa aattataaca tttttaaaac
      241 tttttttttt tgaaagtcct tctcgcttta ggattttttc ccattaagat tatgatggtt
      301 tcatgtatgt gtcagttaaa aaaaatatgg ctaagtagca atgaaacgaa ccaagaaaat
      361 gaaaacaata tatagtgctg ttgaaataac aaagacattg gtatatattt gtaaaatgtc
      421 tgttaaatgt tttttatctc aggttcttgc ttgtctttgg tagaagaaat tatagtaaac
      481 aaacaaacaa acaaacaaaa aaaaaaaaaa aaaaagatct atgtgagacc agaccaataa
      541 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      601 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      661 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      721 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      781 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      841 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
      901 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      961 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     1021 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1081 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1141 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1201 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1261 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1321 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1381 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1861 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2041 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2101 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2161 ggataaccgt
//
