LOCUS       sequence-234842-        2057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet promoter (TetO(x7)-pLEU2m).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS2247
FEATURES             Location/Qualifiers
     source          1..2057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    71..89
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    102..120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    134..152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    165..183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(424..458)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(529..1188)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1103..1122
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1370..1958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1859..1878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgggtag tccatcgttg taggatactc cctatcagtg atagagaaat
       61 ctatgcggca tccctatcag tgatagagat cggtaacgga gtccctatca gtgatagaga
      121 agtggtgttc agttccctat cagtgataga gaagtttgac actatcccta tcagtgatag
      181 agagaacagc aatgactccc tatcagtgat agagatcaaa tggcgtatcc ctatcagtga
      241 tagagataat taacttgtaa tattctaatc aattgacaat attatttaag gacctattgt
      301 tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt
      361 cagcaatata tatatatata tttcaaggat ataccattct aagatctatg tgagaccaga
      421 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      481 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
      541 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
      601 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
      661 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
      721 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
      781 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
      841 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
      901 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
      961 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     1021 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     1081 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     1141 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     1201 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     1261 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt
     1321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1501 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1981 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2041 attctgtgga taaccgt
//
