LOCUS       sequence-234854-        1796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pRC246
FEATURES             Location/Qualifiers
     source          1..1796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(163..197)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(268..927)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     842..861
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1109..1697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1598..1617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc
       61 aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat atatatatat
      121 ttcaaggata taccattcta agatctatgt gagaccagac caataaaaaa cgcccggcgg
      181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      241 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
      301 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      361 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
      421 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
      481 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
      541 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
      601 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
      661 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
      721 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
      781 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
      841 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
      901 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
      961 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     1021 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgt
//
