LOCUS       sequence-234857-        1794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS496
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(161..195)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(266..925)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     840..859
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1107..1695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1596..1615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat aaaactcttg ttttcttctt ttctctaaat attctttcct
       61 tatacattag gacctttgca gcataaatta ctatacttct atagacacac aaacacaaat
      121 acacacacta aattaataag atctatgtga gaccagacca ataaaaaacg cccggcggca
      181 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      241 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      301 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      481 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      601 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      721 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      781 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      841 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      901 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      961 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1021 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgt
//
