LOCUS       sequence-234862-        2661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenosine GPCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS625
FEATURES             Location/Qualifiers
     source          1..2661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1028..1062)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1133..1792)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1707..1726
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1974..2562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2463..2482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgttgtt ggaaacacaa gatgcgttgt atgttgcgtt ggaattggta
       61 attgctgcgc tatcggttgc gggaaatgtt ttggtttgtg ctgcggttgg aacggcgaat
      121 accttgcaaa cgcctactaa ttattttttg gtttcattgg cagcggctga tgttgctgtt
      181 ggactatttg ctattccttt cgctattact atttctttgg gattttgtac cgatttttat
      241 ggatgtctat ttctagcttg ttttgttttg gttctaacgc aatcttcaat tttttctcta
      301 ttggctgttg ccgtagatag gtatttggct atttgcgtac cgctaaggta caagtccctt
      361 gtaacgggaa ctcgtgccag gggagtaata gcagtactat gggtactagc tttcggaatt
      421 ggacttaccc cttttttggg atggaattcc aaggattccg ctactaataa ttgtacagag
      481 ccttgggacg gaactacgaa cgagtcttgt tgtctagtta aatgcctatt cgaaaacgtt
      541 gtacctatgt cttatatggt gtactttaac tttttcggat gcgtgttgcc tcctttgcta
      601 atcatgttgg ttatttatat aaaaattttt ttggttgctt gtaggcaact acaacgtacc
      661 gaattgatgg atcattcgag gactactcta caaagggaaa ttcacgccgc taaatccttg
      721 gctatgatag ttggaatatt cgctttgtgt tggctccctg ttcacgcagt gaattgcgta
      781 accctatttc aacctgcaca aggcaagaac aaacctaaat gggccatgaa catggctata
      841 ctattgtccc acgctaactc cgtggtaaac cctatagtat acgcatatag gaatcgtgat
      901 tttcgttata ccttccataa gataatttca aggtacctac tatgtcaggc cgacgtaaaa
      961 tccggaaacg gccaagcagg agtgcaacct gcactaggcg ttggactcgg atcctgagac
     1021 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1081 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1141 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1201 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1261 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1321 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1381 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1441 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1501 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1561 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1621 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1681 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1741 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1801 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1861 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1921 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2101 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2641 cctgattctg tggataaccg t
//
