LOCUS       sequence-234865-        3088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G_ subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS1037
FEATURES             Location/Qualifiers
     source          1..3088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1455..1489)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1560..2219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2134..2153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2401..2989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2890..2909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatggggtg tacagtgagt acgcaaacaa taggtgacga aagtgatcct
       61 tttctacaga acaaaagagc caatgatgtc atcgagcaat cgttgcagct ggagaaacaa
      121 cgtgacaaga atgaaataaa actgttacta ttaggtgccg gtgagtcagg taaatcaacg
      181 gttttaaaac aattaaaatt attacatcaa ggcggtttct cccatcaaga aaggttacag
      241 tatgctcaag tgatatgggc agatgccata caatcaatga aaattttgat tattcaggcc
      301 agaaaactag gtattcaact tgactgtgat gatccgatca acaataaaga tttgtttgca
      361 tgcaagagaa tactgctaaa ggctaaagct ttagattata tcaacgccag tgttgccggt
      421 ggttctgatt ttctaaatga ttatgtactg aagtactcag aaaggtatga aactaggagg
      481 cgtgttcaga gtaccggacg agcaaaagct gctttcgatg aggacggaaa tatttctaat
      541 gtcaaaagtg acactgacag agatgctgaa acggtgacgc aaaatgagga tgctgataga
      601 aacaacagta gtagaattaa cctacaggat atttgcaagg acttgaacca agaaggcgat
      661 gaccagatgt ttgttagaaa aacatcaagg gaaattcaag gacaaaatag acgaaatctt
      721 attcacgagg acattgctaa ggcaataaag caactttgga ataacgacaa aggtataaag
      781 cagtgttttg cacgttctaa tgagtttcaa ttggagggct cagctgcata ctactttgat
      841 aacattgaga aatttgctag tccgaattat gtctgtacgg atgaggacat tttgaagggc
      901 cgtataaaga ctacaggcat tacagaaacc gaatttaaca tcggctcgtc caaattcaag
      961 gttctcgacg ctggtgggca gcgttctgaa cgtaagaagt ggattcattg tttcgaagga
     1021 attacagcag ttttatttgt tttagcaatg agtgaatacg accagatgtt gtttgaggat
     1081 gaaagagtga acagaatgca tgaatcaata atgctatttg acacgttatt gaactctaag
     1141 tggttcaaag atacaccgtt tattttgttt ttaaataaaa ttgatttgtt cgaggaaaag
     1201 gtaaaaagca tgcccataag aaagtacttt cctgattacc agggacgtgt cggcgatgca
     1261 gaagcgggtc taaaatattt tgagaagata tttttgagct tgaataagac aaacaaacca
     1321 atctacgtga aacgaacctg cgctaccgat acccaaacta tgaagttcgt attgagtgca
     1381 gtcaccgatc taatcatcca gcaaaacctg aaaaaaattg gtattattta actcgagtgg
     1441 ctgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1501 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1561 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1621 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1681 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1741 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     1801 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1861 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1921 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1981 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2041 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2101 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2161 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2221 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2281 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     2341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2521 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3061 gttatcccct gattctgtgg ataaccgt
//
