LOCUS       sequence-234868-        2313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexA bacterial repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS926
FEATURES             Location/Qualifiers
     source          1..2313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(13..264,265..618)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             634..654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(680..714)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(785..1444)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1359..1378
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1626..2214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2115..2134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgaaggc tttaactgca agacaacagg aagtttttga tttgataaga
       61 gatcatatat ctcaaactgg aatgccacca acaagagctg aaatagctca aagattagga
      121 tttagatctc caaacgctgc agaagaacat ttgaaagctt tggcaaggaa aggtgtcatc
      181 gaaattgtct ctggtgcttc aagaggtatt agactattac aagaagagga agaaggattg
      241 ccattggttg gtagagttgc tgctggtgaa ccattattag cacagcaaca tattgagggt
      301 cattatcaag tagatccatc tttattcaaa ccaaatgctg attttttgtt gagagtaagt
      361 ggtatgtcta tgaaagatat tggtatcatg gatggtgact tattggctgt tcataaaact
      421 caagatgtta gaaacggtca agttgtcgtt gccagaattg atgatgaagt cactgttaaa
      481 agattgaaga aacaaggtaa taaagtggaa ttgttaccag aaaattctga atttaagcca
      541 attgttgttg atttaaggca acaatctttt actattgaag gtttagctgt cggtgttatc
      601 agaaatggtg attggttagg ttcaggttct cctccaaaaa aaaagagaaa agttggatct
      661 ggttcttgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      721 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
      781 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      841 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      901 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      961 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1021 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1081 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1141 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1201 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1261 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1321 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1381 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1441 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1501 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     1561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2281 cctgcgttat cccctgattc tgtggataac cgt
//
