LOCUS       sequence-234872-        2379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS033
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(746..780)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(851..1510)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1425..1444
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1692..2280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2181..2200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgtccaa gggtgaagag ctatttactg gggttgtacc cattttggta
       61 gaactggacg gagatgtaaa cggacataaa ttctctgtta gaggtgaggg cgaaggcgat
      121 gccaccaatg gtaaattgac tctgaagttt atatgcacta cgggtaaatt acctgttcct
      181 tggccaaccc tagtaacaac tttgacatat ggtgttcaat gtttctcaag atacccagac
      241 catatgaaaa ggcatgattt ctttaaaagt gctatgccag aaggctacgt gcaagagaga
      301 actatctcct ttaaggatga cggtacgtat aaaacacgag cagaagtgaa attcgaaggg
      361 gatacactag ttaatcgcat cgaattaaag ggtatagact ttaaggaaga tggtaatatt
      421 ctcggccata aacttgagta taatttcaac tcgcataatg tgtacattac agctgacaaa
      481 caaaagaacg gaattaaagc gaattttaaa atcaggcaca acgtcgaaga tgggtctgtt
      541 caacttgccg atcattatca gcaaaacacc cctattggtg atggtccagt cttgttaccc
      601 gataatcact acttaagcac acagtctaga ttgtcaaaag atccgaatga aaagcgtgat
      661 cacatggttt tattggaatt tgtcaccgct gcaggaataa ctcacggaat ggacgagctt
      721 tataagggat cctgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      781 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      841 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      901 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      961 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1021 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     1081 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1141 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1201 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1261 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1321 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1381 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1441 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1501 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1561 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     1621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2341 ttctttcctg cgttatcccc tgattctgtg gataaccgt
//
