LOCUS       sequence-234874-        2167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  _-factor pheromone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS1837
FEATURES             Location/Qualifiers
     source          1..2167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(13..69,70..267,268..279)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
     terminator      complement(534..568)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(639..1298)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1213..1232
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1480..2068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1969..1988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgagatt tccttcaatt tttactgcag ttttattcgc agcatcctcc
       61 gcattagctg ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa
      121 gctgtcatcg gttacttaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc
      181 aacagcacaa ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa
      241 gaagaagggg tatctttgga taaaagagag gctgaagctt ggcattggtt gcaactaaaa
      301 cctggccaac caatgtacaa gagagaagcc gaagctgaag cttggcattg gctgcaacta
      361 aagcctggcc aaccaatgta caaaagagaa gccgacgctg aagcttggca ttggctgcaa
      421 ctaaagcctg gccaaccaat gtacaaaaga gaagccgacg ctgaagcttg gcattggttg
      481 cagttaaaac ccggccaacc aatgtactaa ctcgagtggc tgagaccaga ccaataaaaa
      541 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      601 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      661 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      721 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      781 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      841 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
      901 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      961 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1021 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1081 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1141 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1201 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1261 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1321 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1381 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     1441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1621 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2101 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2161 taaccgt
//
