LOCUS       sequence-234875-        3426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  _-factor protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS788
FEATURES             Location/Qualifiers
     source          1..3426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1793..1827)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1898..2557)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2472..2491
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2739..3327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3228..3247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgtctgc aattaatcat ctttgtttga aacttatttt ggcgagtttc
       61 gcgattatta acaccattac tgctttaaca aacgatggca ctggtcactt agaattcctt
      121 ttacaacacg aagaggagat gtattacgca acaaccttag atataggtac accgtcccaa
      181 agtctgacag tgttgtttga taccggatct gccgattttt gggttatgga ttctagcaat
      241 cccttctgct taccaaattc aaatacgtca tcctattcaa acgcaactta taatggcgaa
      301 gaagttaagc cttcaattga ttgcaggtct atgagtactt ataatgagca tagatcttcc
      361 acctaccaat atctggaaaa tggtaggttt tacatcacat atgctgacgg aacatttgct
      421 gacggtagtt gggggacgga aactgtatca attaatggaa ttgacatccc caatatccag
      481 ttcggagttg ccaagtatgc tacgacaccc gttagtggtg ttcttggaat tgggtttcct
      541 agaagagagt ccgttaaggg ctatgaaggt gctcctaatg aatattatcc taattttcct
      601 cagattttaa aaagtgaaaa aataatcgat gtggtcgcgt attcgctgtt cttaaactca
      661 cctgattcag gtactggttc gattgttttt ggtgccattg atgaatcaaa gttttctggt
      721 gatttgttca ctttccctat ggtaaatgaa tatcccacaa tagtcgacgc tcctgcaact
      781 ttagcaatga ctatacaagg attaggtgcc caaaacaaaa gtagttgtga acatgaaacg
      841 tttacgacga ccaagtatcc agttttgttg gactcaggaa cctcgctatt gaatgcgccc
      901 aaggtcatag cagataaaat ggcttctttt gtaaatgcgt cctatagtga agaggaaggt
      961 atatatatat tagactgtcc agtatctgta ggtgacgtgg aatacaattt tgatttcggc
     1021 gatttgcaaa taagtgttcc actgtctagt ttgattttaa gtcccgagac agaaggcagc
     1081 tattgtgggt ttgcggtcca gccaacaaac gattcgatgg ttctgggtga tgtgttcctg
     1141 tcctctgcat acgtcgtatt cgatctcgat aattataaga tatctttagc acaggcaaat
     1201 tggaacgcaa gcgaagtttc gaaaaagcta gtaaatattc aaacagatgg gtctatttca
     1261 ggtgccaaaa ttgctacagc tgaaccctgg tccaccaatg aaccatttac agtcacctct
     1321 gacatttatt catctacagg ctgcaagagt aggccttttc ttcaatcatc gacagcctct
     1381 tcgcttattg cagaaaccaa cgtacaaagt cgcaactgct ctacgaagat gccaggcact
     1441 agatcaacta ctgtcttaag taagcctact caaaatagtg ctatgcatca aagtacaggc
     1501 gctgtcacac aaacctcaaa tgaaactaaa ttagaattat cctcgactat ggcaaattcg
     1561 ggcagtgtct cgcttcccac ttcgaattca atagacaaag agttcgaaca ttcgaaatct
     1621 caaactacca gcgatccaag tgtagcagag cattctacgt ttaaccaaac gtttgtacat
     1681 gaaactaaat atcggcctac tcataagaca gtcataacag aaactgtcac gaagtattct
     1741 acagtcttaa taaatgtctg taaaccaaca tatggatcct gagaccagac caataaaaaa
     1801 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1861 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1921 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1981 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2041 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2101 aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat
     2161 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2221 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2281 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2341 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2401 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2461 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2521 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2581 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2641 aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3421 aaccgt
//
