LOCUS       sequence-23492-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ham TS, Lee SK, Keasling JD, Arkin AP
  TITLE     A tightly regulated inducible expression system utilizing the fim 
            inversion recombination switch.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):1-4.
  PUBMED    16534780
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):1-4."
COMMENT     Sequence Label: pFIP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     775..794
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        775..793
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggactnctga gcttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta
       61 gcagggacag gatatgtgag taaacgtcgt tatcttaccg gtaaagaagt tcaggccatg
      121 atgcaggcgg tttgttacgg ggcaacggga gccagagatt attgtcttat tctgttggca
      181 tatcggcatg ggatgcgtat tagtgaactg cttgatctgc attatcagga ccttgacctt
      241 aatgaaggta gaataaatat tcgccgactg aagaacggat tttctaccgt tcacccgtta
      301 cgttttgatg agcgtgaagc cgtggaacgc tggacccagg aacgtgctaa ctggaaaggc
      361 gctgaccgga ctgacgctat atttatttct cgccgcggga gtcggctttc tcgccagcag
      421 gcctatcgca ttattcgcga tgccggtatt gaagctggaa ccgtaacgca gactcatcct
      481 catatgttaa ggcatgcttg cggttatgaa ttggcggagc gtggtgcaga tactcgttta
      541 attcaggatt atctcgggca tcgaaatatt cgccatactg tgcgttatac cgccagtaat
      601 gctgctcgtt ttgccggatt atgggaaaga aataatctca taaacgaaaa attaaaaaga
      661 gaagaggttt gatannannc gnnnccncgn nnncnncngc attggaatna agnttgggta
      721 acgccagggt tttccnagtc acgacgttgt aaancgacgn ccagtgagcg cgcgtaatac
      781 gactcactat agggcgaatt ggagctgggg ccgcaattcc aattcccngg catcaaanaa
      841 acgaaggc
//
