LOCUS       sequence-234925-       11431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX311 LacZ
FEATURES             Location/Qualifiers
     source          1..11431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1321..1362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(1373..1397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1405..1446
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1497..2085
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1550..1570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1968..1979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2157..2390
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2468..2589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2505..2524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2559..2578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2629..2764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2784..2803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2785..2803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2813..2830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2813..2829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2971..3426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3058..3077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3268..3289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3452..3556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3557..3625,3626..4417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3775..3794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4588..5176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5077..5096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5330..5347
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5464..5485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5500..5505,5506..5523,5524..5530)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5538..5554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5543..5565
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5562..5578
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5562..5578
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5597..5617
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5599..5617
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5645..5871
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5872..6052
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6099..6224
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6717..6950
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7135..7179
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7410..7620
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7432..7628
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7479..7614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7650..8045
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8119..8236
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8374..9552
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8605..9543
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9500..9520
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    9572..9596
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9611..11431,1..1224)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
ORIGIN
        1 gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc
       61 atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg
      121 gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa
      181 ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg
      241 ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg ccacatcagc
      301 gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac
      361 gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt
      421 tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa
      481 ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta
      541 agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat
      601 taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga cgcggtgctg
      661 attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc
      721 taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat
      781 acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta
      841 aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt
      901 gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac
      961 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
     1021 cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg
     1081 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtgga
     1141 gacgactcct ggagcccgtc agtatcggcg gaattacagc tgagcgccgg tcgctaccat
     1201 taccagttgg tctggtgtca aaaaactaag ggtgggcgcg ccgacccagc tttcttgtac
     1261 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     1321 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tttgccaact
     1381 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1441 tctacgtagt aatgaactag taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc
     1501 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     1801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1861 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     1921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     1981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     2101 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2161 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2221 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2281 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2341 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     2401 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     2461 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2581 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     2641 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2701 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2761 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     2821 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2881 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2941 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     3001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3361 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3421 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3541 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3601 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3661 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3721 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3781 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3841 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3901 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3961 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4021 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4081 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4141 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4201 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4261 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4321 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4381 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4441 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4501 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4561 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4621 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4681 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4741 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4801 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4861 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4921 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4981 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5041 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5101 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5161 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5221 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5281 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5341 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5401 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5461 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5521 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5581 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     5641 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     5701 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     5761 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     5821 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     5881 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5941 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6001 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     6061 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     6121 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6181 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6241 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6301 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6361 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6421 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6481 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6541 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     6601 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6661 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     6721 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     6781 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     6841 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     6901 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     6961 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     7021 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     7081 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     7141 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     7201 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7261 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7321 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     7381 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     7441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7561 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7621 tttgcaaaaa gctttctaga ggtaccacca tggccaagcc tttgtctcaa gaagaatcca
     7681 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     7741 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     7801 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     7861 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     7921 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     7981 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     8041 agggcctgca gctgcagtag taaggcgcgc cgttaacgaa ttctagatct tgagacaaat
     8101 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     8161 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     8221 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     8281 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     8341 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     8401 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     8461 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     8521 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     8581 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     8641 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     8701 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     8761 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     8821 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     8881 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     8941 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     9001 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     9061 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     9121 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     9181 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     9241 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     9301 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     9361 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     9421 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     9481 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     9541 caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag ttggcaccat
     9601 gatagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
     9781 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
     9841 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
     9901 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
     9961 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
    10021 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
    10081 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
    10141 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
    10201 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
    10261 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
    10321 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
    10381 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
    10441 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
    10501 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
    10561 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
    10621 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
    10681 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
    10741 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
    10801 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
    10861 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
    10921 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
    10981 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
    11041 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
    11101 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
    11161 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
    11221 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
    11281 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
    11341 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
    11401 tctggtcttt gccgaccgca cgccgcatcc a
//
