LOCUS       sequence-234927-        9178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding DEDD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX311 DEDD
FEATURES             Location/Qualifiers
     source          1..9178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             179..574
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    648..765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        903..2081
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1134..2072
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2029..2049
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2101..2125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3080..3104)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3112..3153
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3204..3792
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3257..3277)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3675..3686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3864..4097
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4175..4296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4212..4231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4266..4285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4336..4471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4491..4510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4492..4510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4520..4537)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4520..4536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4529..4551)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4678..5133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4765..4784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4975..4996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5159..5263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5264..5332,5333..6124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5482..5501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6295..6883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6784..6803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7037..7054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7207..7212,7213..7230,7231..7237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7250..7272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7269..7285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7269..7285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7304..7324
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7306..7324
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7352..7578
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7579..7759
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7806..7931
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8424..8657
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8842..8886
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(9117..9178,1..149)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        join(9139..9178,1..157)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
       61 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccat
      181 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
      241 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
      301 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
      361 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
      421 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
      481 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
      541 tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aaggcgcgcc
      601 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
      661 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      721 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      781 gacagcagag atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt
      841 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      901 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      961 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     1021 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     1081 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     1141 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     1201 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     1261 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     1321 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     1381 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     1441 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     1501 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     1561 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     1621 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     1681 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     1741 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1801 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1861 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1921 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1981 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     2041 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggctagcat cgattgatca
     2101 acaagtttgt acaaaaaagt tggcatggcg ggcctaaagc ggcgggcaag ccaggtgtgg
     2161 ccagaagagc atggtgagca ggaacatggg ctgtacagcc tgcaccgcat gtttgacatc
     2221 gtgggcactc atctgacaca cagagatgtg cgcgtgcttt ctttcctctt tgttgatgtc
     2281 attgatgacc acgagcgtgg actcatccga aatggacgtg acttcttatt ggcactggag
     2341 cgccagggcc gctgtgatga aagtaacttt cgccaggtgc tgcagctgct gcgcatcatc
     2401 actcgccacg acctgctgcc ctacgtcacc ctcaagagga gacgggctgt gtgccctgat
     2461 cttgtagaca agtatctgga ggagacatca attcgctatg tgacccccag agccctcagt
     2521 gatccagaac caaggcctcc ccagccctct aaaacagtgc ctccccacta tcctgtggtg
     2581 tgttgcccca cttcgggtcc tcagatgtgt agcaagcggc cagcccgagg gagagccaca
     2641 cttgggagcc agcgaaaacg ccggaagtca gtgacaccag atcccaagga gaagcagaca
     2701 tgtgacatca gactgcgggt tcgggctgaa tactgccagc atgagactgc tctgcagggc
     2761 aatgtcttct ctaacaagca ggacccactt gagcgccagt ttgagcgctt taaccaggcc
     2821 aacaccatcc tcaagtcccg ggacctgggc tccatcatct gtgacatcaa gttctctgag
     2881 ctcacctacc tcgatgcatt ctggcgtgac tacatcaatg gctctttatt agaggcactt
     2941 aaaggtgtct tcatcacaga ctccctcaag caagctgtgg gccatgaagc catcaagctg
     3001 ctggtaaatg tagacgagga ggactatgag ctgggccgac agaaactcct gaggaacttg
     3061 atgctgcaag cattgccctg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     3121 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtac cggttaagtc
     3181 gacaatcaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3601 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3781 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     3841 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3901 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3961 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4021 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4081 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     4141 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     4201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     4321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4441 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     4501 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4561 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4621 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5101 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     5161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7261 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     7321 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     7381 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     7441 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7501 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7561 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7621 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7681 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7741 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7801 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7861 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     7921 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     7981 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8041 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8101 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8161 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8221 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8281 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8341 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8401 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8461 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8521 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8581 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8641 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8701 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8761 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8821 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8881 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8941 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9001 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9061 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9121 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaac
//
