LOCUS       sequence-234940-       10066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A
            and an antibiotic resistance cassette (zeocin). Used for selection 
            of endothelial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10066)
  TITLE     CDH5-GFP-AB
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CDH5-AbR-GFP
FEATURES             Location/Qualifiers
     source          1..10066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1951..1971
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3468..3492
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3513..4178
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             3513..4178
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             4320..4373
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(4774..4798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4851..4892
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4916..5504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4969..4989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5387..5398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5523..6022
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5558..5581)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5955..5974
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6036..6083
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6102..6500
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6587..6820
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6892..7013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6929..6948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6983..7002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7053..7188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7208..7227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7209..7227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7237..7254)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7237..7253)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7246..7268)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7395..7850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7482..7501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7692..7713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7876..7980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7981..8049,8050..8841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8199..8218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9012..9600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9501..9520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9754..9771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9888..9909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9924..9929,9930..9947,9948..9954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9962..9978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9967..9989
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9986..10002
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9986..10002
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10021..10041
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10023..10041
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatgtc gacgttaacg ctagtgatat caactttgta tagaaaagtt ggctccgaat
     1981 tcgcccttca aatgtcagag ggtccaagtc ctccctccct ccaagctcat ccatgcccat
     2041 ggcctcagat gccagccata agctgttggg ttccaaacct cgactccagg ctggactcac
     2101 ccctgtctcc cccaccagcc tgacacctcc acctgggtat ctaacgagca tctcaaactc
     2161 aacctgcctg agacagagga atcactatcc cctcctcctc caaaaatatc cttccatcac
     2221 actccccatc ttgtgctctg atttactaaa cggccctggg ccctctcttt ctcagggtct
     2281 ctgcttgccc agctatataa taaaacaagt ttgggacttc ccaaccattc acccatggaa
     2341 aaacagaagc aactcttcaa aggacagatt cccaggatct gccctgggag attccaaatc
     2401 agttgatctg gggtgagccc agtcctctgt agtttttaga agctcctcct atgtctctcc
     2461 tggtcagcag aatcttggcc cctcccttcc ccccagcctc ttggttcttc tgggctctga
     2521 tccagcctca gcgtcactgt cttccacgcc cctctttgat tctcgtttat gtcaaaagcc
     2581 ttgtgaggat gaggctgtga ttatccccat tttacagatg aggaaactgt ggctccagga
     2641 tgacacaact ggccagaggt cacatcagaa gcagagctgg gtcacttgac tccacccaat
     2701 atccctaaat gcaaacatcc cctacagacc gaggctggca ccttagagct ggagtccatg
     2761 cccgctctga ccaggagaag ccaacctggt cctccagagc caagagcttc tgtccctttc
     2821 ccatctcctg aagcctccct gtcaccttta aagtccattc ccacaaagac atcatgggat
     2881 caccacagaa aatcaagctc tggggctagg ctgaccccag ctagattttt ggctctttta
     2941 taccccagct gggtggacaa gcaccttaaa cccgctgagc ctcagcttcc cgggctataa
     3001 aatgggggtg atgacacctg cctgtagcat tccaaggagg gttaaatgtg atgctgcagc
     3061 caagggtccc cacagccagg ctctttgcag gtgctgggtt cagagtccca gagctgaggc
     3121 cgggagtagg ggttcaagtg gggtgcccca ggcagggtcc agtgccagcc ctctgtggag
     3181 acagccatcc ggggccgagg cagccgccca ccgcagggcc tgcctatctg cagccagccc
     3241 agccctcaca aaggaacaat aacaggaaac catcccaggg ggaagtgggc cagggccagc
     3301 tggaaaacct gaaggggagg cagccaggcc tccctcgcca gcggggtgtg gctcccctcc
     3361 aaagacggtc ggctgacagg ctccacagag ctccactcac gctcagccct ggacggacag
     3421 gcagtccaac ggaacagaaa catccctcag ccaagggcga attcgaccca agtttgtaca
     3481 aaaaagcagg ctccgcggcc gcccccttca ccatgcccgc catgaagatc gagtgccgca
     3541 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcacccccg
     3601 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
     3661 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
     3721 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
     3781 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
     3841 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg
     3901 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc
     3961 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg
     4021 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
     4081 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg
     4141 agtaccagca cgccttcaag acccccatcg ccttcgccag atctcgagat atcagccatg
     4201 gcttcccgcc ggcggtggcg gcgcaggatg atggcacgct gcccatgtct tgtgcccagg
     4261 agagcgggat ggaccgtcac cctgcagcct gtgcttctgc taggatcaat gtgaccggtg
     4321 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc ccttccggta
     4381 tggccaaact cacttctgca gtcccagtcc tcacagcaag ggatgttgca ggggctgtag
     4441 agttctggac tgacagatta ggattctcca gagactttgt tgaagatgat tttgctggtg
     4501 ttgtcagaga tgatgtcacc ctcttcatct cagcagttca ggaccaagtt gtccctgaca
     4561 acacccttgc ttgggtctgg gtcagaggcc tagatgagct ttatgcagaa tggtcagaag
     4621 tagtcagcac aaatttcagg gatgcctctg gcccagccat gacagaaatt ggtgaacaac
     4681 cttggggaag ggaatttgcc ctcagagacc ctgctggaaa ttgtgtccat tttgtagctg
     4741 aggaacagga ctagtcgaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg
     4801 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
     4861 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga tcgacaatca
     4921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5281 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     5341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggcg atggtaccta
     5521 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     5581 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     5641 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     5701 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     5761 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     5821 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     5881 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     5941 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     6001 ttcctcatct ccgggccttt cgactctaga cacgtgttga caattaatca tcggcatagt
     6061 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc
     6121 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     6181 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     6241 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     6301 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     6361 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     6421 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     6481 gttatgtgtg ggagggctaa gcacaattcg agctcggtac ctttaagacc aatgacttac
     6541 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6601 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6841 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     6901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7021 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     7201 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     7261 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     7321 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7381 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7441 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7501 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7561 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7621 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7681 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7741 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7801 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     7861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8101 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8161 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8221 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8281 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8341 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8401 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8461 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8521 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8581 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8641 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8701 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8761 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8821 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8881 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8941 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9901 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9961 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
    10021 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagctt
//
