LOCUS       sequence-234947-        9364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding DEDD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX307 DEDD
FEATURES             Location/Qualifiers
     source          1..9364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             166..768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(166..185)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     662..682
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    811..928
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1066..2244
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1297..2235
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2192..2212
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2264..2288
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3243..3267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3275..3316
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3367..3955
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3420..3440)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3838..3849)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4027..4260
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4338..4459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4375..4394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4429..4448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4499..4634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4654..4673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4655..4673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4683..4700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4683..4699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4692..4714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4841..5296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4928..4947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5138..5159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5322..5426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5427..5495,5496..6287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5645..5664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6458..7046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6947..6966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7200..7217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7334..7355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7370..7375,7376..7393,7394..7400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7408..7424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7413..7435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7432..7448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7432..7448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7467..7487
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7469..7487
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7515..7741
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7742..7922
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7969..8094
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8587..8820
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9005..9049
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(9280..9364,1..126)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        join(9302..9364,1..134)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      join(9349..9364,1..120)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
       61 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      121 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
      181 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
      241 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
      301 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
      361 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
      421 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
      481 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
      541 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
      601 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
      661 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
      721 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
      781 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      841 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      901 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      961 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     1021 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     1081 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     1141 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     1201 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     1261 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     1321 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     1381 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     1441 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     1501 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     1561 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     1621 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     1681 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     1741 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     1801 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     1861 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1921 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1981 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     2041 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     2101 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     2161 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     2221 tttcttccat ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa
     2281 agttggcatg gcgggcctaa agcggcgggc aagccaggtg tggccagaag agcatggtga
     2341 gcaggaacat gggctgtaca gcctgcaccg catgtttgac atcgtgggca ctcatctgac
     2401 acacagagat gtgcgcgtgc tttctttcct ctttgttgat gtcattgatg accacgagcg
     2461 tggactcatc cgaaatggac gtgacttctt attggcactg gagcgccagg gccgctgtga
     2521 tgaaagtaac tttcgccagg tgctgcagct gctgcgcatc atcactcgcc acgacctgct
     2581 gccctacgtc accctcaaga ggagacgggc tgtgtgccct gatcttgtag acaagtatct
     2641 ggaggagaca tcaattcgct atgtgacccc cagagccctc agtgatccag aaccaaggcc
     2701 tccccagccc tctaaaacag tgcctcccca ctatcctgtg gtgtgttgcc ccacttcggg
     2761 tcctcagatg tgtagcaagc ggccagcccg agggagagcc acacttggga gccagcgaaa
     2821 acgccggaag tcagtgacac cagatcccaa ggagaagcag acatgtgaca tcagactgcg
     2881 ggttcgggct gaatactgcc agcatgagac tgctctgcag ggcaatgtct tctctaacaa
     2941 gcaggaccca cttgagcgcc agtttgagcg ctttaaccag gccaacacca tcctcaagtc
     3001 ccgggacctg ggctccatca tctgtgacat caagttctct gagctcacct acctcgatgc
     3061 attctggcgt gactacatca atggctcttt attagaggca cttaaaggtg tcttcatcac
     3121 agactccctc aagcaagctg tgggccatga agccatcaag ctgctggtaa atgtagacga
     3181 ggaggactat gagctgggcc gacagaaact cctgaggaac ttgatgctgc aagcattgcc
     3241 ctgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     3301 cggtctcgat tctacgtagt aatgaactag taccggttaa gtcgacaatc aacgcgttaa
     3361 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3421 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3481 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3541 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3601 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3661 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3721 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3781 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3841 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3901 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3961 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4021 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4261 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4321 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4381 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4441 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4501 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4561 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4621 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4681 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4741 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4801 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5281 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5341 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5401 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5521 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5581 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5641 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5701 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5821 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5941 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6001 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6301 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7141 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7201 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7261 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7321 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7381 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7441 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7501 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7561 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7621 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7681 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7741 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7801 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7861 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7921 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7981 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8041 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8101 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8161 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8221 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8281 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8341 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8401 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8461 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8521 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8581 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8641 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8701 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8761 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8821 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8881 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8941 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9001 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9061 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9121 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9181 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9241 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9301 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9361 actc
//
